Related Experiment Video
Updated: Mar 22, 2026

A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization
Published on: July 6, 2009
Standards in reporting cryopreserved donor sperm characteristics: should they be reported post-thaw or post-wash?
Mathew J Tomlinson1, Estel Comajuan2, Asad Naeem3
1Fertility Unit, East block B Floor, Nottingham University Hospital, Derby Road, Nottingham, UK; School of Medicine, Division Child Health, Obstetrics and Gynaecology, D Floor, East Block, Queen's Medical Centre, Nottingham, UK.
Abstract:
An increase in the reliance on imported donor samples has been the consequence of a continued shortage of UK donors. Disputes can arise between suppliers and purchasers if the sperm quality is not as expected, yet there appears to be no requirement for the standardization of methods for sperm processing or analysis. Following analysis of 102 donor intrauterine insemination cycles, this study demonstrates that the motile sperm concentration is significantly (P < 0.05) reduced after the necessary removal of cryoprotectant before insemination. Suppliers of donor spermatozoa should therefore provide information on standards used for sperm assessment and whether analysis is performed before or after washing in order that purchasers are better informed about the quality of the end product they are committed to buying.

