Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

4.5K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
4.5K
Intracellular Movement of Viruses and Bacteria01:10

Intracellular Movement of Viruses and Bacteria

3.8K
Intracellular bacteria and viruses often comprise a group of highly infectious pathogens that can cause several diseases. Bacterial pathogens include those belonging to the genus Rickettsia responsible for conditions such as rocky mountain spotted fever and the Mediterranean spotted fever; Chlamydia, a genus responsible for a sexually transmitted disease; Coxiella burnetii, an agent responsible for Q fever. Viral pathogens include vaccinia—a poxvirus, and herpes simplex virus—a...
3.8K
Viral Structure00:56

Viral Structure

75.7K
Viruses are extraordinarily diverse in shape and size, but they all have several structural features in common. All viruses have a core that contains a DNA- or RNA-based genome. The core is surrounded by a protective coat of proteins called the capsid. The capsid is composed of subunits called capsomeres. The capsid and genome-containing core are together known as the nucleocapsid.
75.7K
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

3.0K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
3.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Unraveling the maturation pathway of a eukaryotic virus through cryo-EM.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

ViReMa: a virus recombination mapper of next-generation sequencing data characterizes diverse recombinant viral nucleic acids.

GigaScience·2023
Same author

Plant-expressed virus-like particles reveal the intricate maturation process of a eukaryotic virus.

Communications biology·2021
Same author

Icosahedral virus structures and the protein data bank.

The Journal of biological chemistry·2021
Same author

Dynamics and stability in the maturation of a eukaryotic virus: a paradigm for chemically programmed large-scale macromolecular reorganization.

Archives of virology·2021
Same author

Vitamin C mediates the activation of green tea extract to modify nanozero-valent iron composites: Enhanced transport in heterogeneous porous media and the removal of hexavalent chromium.

Journal of hazardous materials·2021

Related Experiment Video

Updated: Mar 22, 2026

Advancing High-Resolution Imaging of Virus Assemblies in Liquid and Ice
08:31

Advancing High-Resolution Imaging of Virus Assemblies in Liquid and Ice

Published on: July 20, 2022

3.8K

Virus particle dynamics derived from CryoEM studies.

Peter C Doerschuk1, Yunye Gong2, Nan Xu2

  • 1Biomedical and Electrical and Computer Engineering, Cornell University, Ithaca, NY, United States.

Current Opinion in Virology
|April 18, 2016
PubMed
Summary
This summary is machine-generated.

Direct electron detectors in electron cryo-microscopy (CryoEM) reveal virus dynamics. This review explores capturing dynamic viral information from CryoEM data, offering insights into particle maturation.

More Related Videos

Averaging of Viral Envelope Glycoprotein Spikes from Electron Cryotomography Reconstructions using Jsubtomo
08:29

Averaging of Viral Envelope Glycoprotein Spikes from Electron Cryotomography Reconstructions using Jsubtomo

Published on: October 21, 2014

12.7K
Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction
12:38

Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction

Published on: August 9, 2011

17.9K

Related Experiment Videos

Last Updated: Mar 22, 2026

Advancing High-Resolution Imaging of Virus Assemblies in Liquid and Ice
08:31

Advancing High-Resolution Imaging of Virus Assemblies in Liquid and Ice

Published on: July 20, 2022

3.8K
Averaging of Viral Envelope Glycoprotein Spikes from Electron Cryotomography Reconstructions using Jsubtomo
08:29

Averaging of Viral Envelope Glycoprotein Spikes from Electron Cryotomography Reconstructions using Jsubtomo

Published on: October 21, 2014

12.7K
Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction
12:38

Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction

Published on: August 9, 2011

17.9K

Area of Science:

  • Structural Biology
  • Biophysics
  • Virology

Background:

  • Direct electron detectors have transformed electron cryo-microscopy (CryoEM), enabling high-resolution icosahedral virus structure determination.
  • CryoEM now often replaces virus crystallography due to lower material requirements, reduced purity needs, and faster structure acquisition.
  • The dynamic information present in CryoEM datasets has been largely overlooked.

Purpose of the Study:

  • To review an approach for capturing dynamic viral characteristics from CryoEM data.
  • To highlight the potential of CryoEM for studying virus particle dynamics.
  • To illustrate the application of this approach in understanding virus particle maturation.

Main Methods:

  • Utilizing the ensemble of particles in CryoEM data captured at the moment of freezing.
  • Illustrating the methodology with a simplified model.
  • Describing the technical details of the dynamic information extraction process.

Main Results:

  • Demonstration of an approach to extract dynamic information from existing CryoEM datasets.
  • Successful application of the method to study virus particle maturation.
  • Highlighting previously inaccessible dynamic insights into viral structures.

Conclusions:

  • The dynamic character of viruses can be effectively captured from CryoEM data.
  • This approach offers a powerful complement to static structural information obtained from CryoEM.
  • Further exploration of dynamic information in CryoEM holds significant potential for virology research.