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Related Experiment Video

Updated: Mar 22, 2026

Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors
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A logic gate-based fluorogenic probe for Hg(2+) detection and its applications in cellular imaging.

Jiwen Hu1, Zhangjun Hu2, Zhiwen Chen3

  • 1State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai, 200092, PR China; Division of Molecular Surface Physics & Nanoscience, Department of Physics, Chemistry and Biology, Linköping University, Linköping, 58183, Sweden.

Analytica Chimica Acta
|April 18, 2016
PubMed
Summary
This summary is machine-generated.

A novel rhodamine-based probe (RN3) selectively detects mercury ions (Hg2+) with a visible color change and enhanced fluorescence. This allows for sensitive mercury detection and potential use in cellular imaging and logic gate applications.

Keywords:
Fluorescent imagingFluorogenic probeLogical gateMercuryRhodamine

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Area of Science:

  • Chemical Sensing
  • Fluorescent Probes
  • Analytical Chemistry

Background:

  • Mercury ions (Hg2+) pose significant environmental and health risks.
  • Developing selective and sensitive probes for Hg2+ detection is crucial.
  • Rhodamine-based compounds are widely used as fluorescent platforms.

Purpose of the Study:

  • To design and synthesize a new colorimetric and fluorogenic probe (RN3) for selective Hg2+ detection.
  • To investigate the sensing mechanism and performance of RN3.
  • To explore the application of RN3 in cellular imaging and logic gate construction.

Main Methods:

  • Synthesis of RN3 probe based on rhodamine-B.
  • Spectrophotometric and fluorometric analysis of RN3 response to metal ions.
  • Job's plot analysis for binding stoichiometry determination.
  • Evaluation of RN3 for cellular imaging and logic gate applications.

Main Results:

  • RN3 exhibited selective colorimetric and fluorogenic response to Hg2+.
  • A 105-fold fluorescence enhancement and visible color change (colorless to red) were observed upon Hg2+ binding.
  • The probe demonstrated a 1:1 binding stoichiometry with Hg2+.
  • RN3 allowed for sensitive Hg2+ determination with a limit of detection of 60.7 nM.
  • Reversible switching and INHIBIT logic gate behavior were observed.
  • Successful application in cellular imaging was demonstrated.

Conclusions:

  • RN3 is a highly selective and sensitive fluorescent probe for Hg2+ detection.
  • The probe's mechanism involves Hg2+-induced spirolactam ring-opening.
  • RN3 shows potential for environmental monitoring, biological sensing, and constructing molecular logic gates.