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Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution
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Defining and improving the genome-wide specificities of CRISPR-Cas9 nucleases

Shengdar Q Tsai1, J Keith Joung1

  • 1Molecular Pathology Unit, Center for Cancer Research, and Center for Computational and Integrative Biology, Massachusetts General Hospital, 149 13th Street, Charlestown, Massachusetts 02129, USA; and the Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA.

Nature Reviews. Genetics
|April 19, 2016
PubMed

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