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Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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Thermochemical Studies of NiII and ZnII Ternary Complexes Using Ion Mobility-Mass Spectrometry
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Nanotip Ambient Ionization Mass Spectrometry.

Zhenpeng Zhou1, Jae Kyoo Lee1, Samuel C Kim1

  • 1Department of Chemistry, Stanford University , Stanford, California 94305-5080, United States.

Analytical Chemistry
|April 19, 2016
PubMed
Summary

A novel nanotip ambient ionization mass spectrometry (NAIMS) technique offers high sensitivity and spatial resolution for analyzing surface analytes. This method optimizes plasma generation for improved ionization efficiency and detection limits.

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Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry
  • Surface Science

Background:

  • Direct analysis of surface analytes is challenging.
  • Existing ambient ionization techniques have limitations in sensitivity or spatial resolution.

Purpose of the Study:

  • To introduce and characterize a new technique: nanotip ambient ionization mass spectrometry (NAIMS).
  • To optimize NAIMS parameters for enhanced sensitivity and spatial resolution.
  • To demonstrate the capabilities of NAIMS for analyzing surface samples.

Main Methods:

  • High voltage applied between a tungsten nanotip and a metal plate to generate plasma.
  • Optimization of tip-to-plate distance, tip size, voltage, and current.
  • Utilized a partially observable Markov decision process for plasma stabilization and ionization efficiency.

Main Results:

  • Achieved low limits of detection: 0.14 fmol for phenanthrene and 4 amol for caffeine.
  • Demonstrated high spatial resolution, with a best resolution of 5 μm for a printed caffeine pattern.
  • Investigated and optimized key parameters influencing signal intensity.

Conclusions:

  • NAIMS is a promising technique for sensitive and high-resolution surface analysis.
  • The method offers significant advantages over existing ambient ionization techniques.
  • Further exploration of NAIMS limitations and applications is warranted.