Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

External Acoustic Meatus Approach for Ultrasonographic Evaluation of the Temporomandibular Joint: An Anatomical Validation and Proof-of-Concept Study.

Clinical anatomy (New York, N.Y.)·2026

Same author

Author Correction: Mitochondrial fission links ECM mechanotransduction to metabolic redox homeostasis and metastatic chemotherapy resistance.

Nature cell biology·2026

Same author

Investigating Intraspecific Attacks in Brown Bears (<i>Ursus arctos</i>) Using a Forensic Approach: Evidence from Northern Italy.

Animals : an open access journal from MDPI·2026

Same author

Effects of Human Recombinant Growth Hormone (rhGH) Treatment on Plasma Extracellular Vesicles in GH-Deficient Children: A Preliminary Report.

Journal of clinical medicine·2026

Same author

Combination of hypoxia and hyperosmolarity reduces <i>in vitro</i> chondrocyte de-differentiation.

Frontiers in veterinary science·2026

Same author

Correction: Tonon et al. 5-Azacytidine Downregulates the Proliferation and Migration of Hepatocellular Carcinoma Cells In Vitro and In Vivo by Targeting miR-139-5p/ROCK2 Pathway. <i>Cancers</i> 2022, <i>14</i>, 1630.

Cancers·2026

Same journal

Efficient evidence-based genome annotation with EviAnn.

Nature methods·2026

Same journal

ClairS: a deep-learning method for long-read tumor-normal pair somatic small variant calling.

Nature methods·2026

Same journal

RNAbpFlow: base pair-augmented SE(3) flow matching for conditional RNA 3D structure generation.

Nature methods·2026

Same journal

Spatio-DARLIN enables robust and efficient in situ lineage tracing in mice at single-cell resolution.

Nature methods·2026

Same journal

EasyGrid: a versatile platform for automated cryo-EM sample preparation and quality control.

Nature methods·2026

Same journal

Cloud-based microscope enables live neuroimaging for 24 h and beyond with worldwide access.

Nature methods·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Mar 22, 2026

Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device

Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device

Published on: April 17, 2021

High-efficiency cellular reprogramming with microfluidics.

Camilla Luni^1,2, Stefano Giulitti^1,2, Elena Serena^1,2

¹Department of Industrial Engineering, University of Padova, Padova, Italy.

|April 19, 2016

Summary

This summary is machine-generated.

Microfluidic technology significantly boosts reprogramming efficiency for human induced pluripotent stem cells (hiPSCs), achieving a 50-fold increase. This method yields high-quality hiPSCs suitable for direct differentiation into specific cell types.

More Related Videos

Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform

Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform

Published on: November 7, 2013

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

Published on: June 16, 2016

Related Experiment Videos

Last Updated: Mar 22, 2026

Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device

Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device

Published on: April 17, 2021

Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform

Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform

Published on: November 7, 2013

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

Published on: June 16, 2016

Area of Science:

Stem Cell Biology
Microfluidics
Cellular Reprogramming

Background:

Induced pluripotent stem cells (hiPSCs) hold promise for regenerative medicine.
Traditional hiPSC generation methods, often using mRNA delivery, face efficiency limitations.
Optimizing reprogramming conditions is crucial for generating clinical-grade stem cells.

Purpose of the Study:

To investigate the impact of microfluidic environments on human somatic cell reprogramming efficiency.
To characterize the quality and differentiation potential of hiPSCs generated in microfluidic systems.

Main Methods:

Reprogramming of human somatic cells using synthetic mRNAs encoding transcription factors within a microfluidic device.
Microliter-volume confinement to alter extracellular signaling environments.
Analysis of reprogramming efficiency, hiPSC quality, and subsequent directed differentiation.

Main Results:

A 50-fold increase in hiPSC reprogramming efficiency was observed in the microfluidic environment compared to traditional methods.
Temporal regulation of TGF-β and other signaling pathways was identified as critical for pluripotency acquisition.
High-quality, pure hiPSCs (μ-hiPSCs) were generated, enabling direct differentiation into hepatocyte- and cardiomyocyte-like cells without further expansion.

Conclusions:

Microfluidic technology offers a highly efficient platform for generating hiPSCs.
Controlled microenvironments can optimize stem cell reprogramming by modulating signaling pathways.
This platform facilitates the generation of clinical-ready hiPSCs for direct therapeutic applications.