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Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
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siFLIM: single-image frequency-domain FLIM provides fast and photon-efficient lifetime data
Marcel Raspe1, Katarzyna M Kedziora1, Bram van den Broek1
1Department of Cell Biology, The Netherlands Cancer Institute, Amsterdam, the Netherlands.
Nature Methods
|April 19, 2016
Abstract:
We developed single-image fluorescence lifetime imaging microscopy (siFLIM), a method for acquiring quantitative lifetime images from a single exposure. siFLIM takes advantage of a new generation of dedicated cameras that simultaneously record two 180°-phase-shifted images, and it allows for video-rate lifetime imaging with minimal phototoxicity and bleaching. siFLIM is also inherently immune to artifacts stemming from rapid cellular movements and signal transients.

