A comparison of two techniques for the quantitative determination of oocysts in faecal samples

Coccidial oocysts (Eimeria tenella) were counted in faecal samples (n = 2 * 101) from two broiler anticoccidial battery trials. A direct flotation technique using zinc sulphate solution and an indirect technique using sodium chloride solution for the examination of samples stored in potassium dichromate were compared. Both techniques were correlated (P less than 0.001) and showed little difference in sensitivity. With the indirect method, oocyst counts were slightly lower (-1.53%), but the difference between the experimental groups was not significant (Wilcoxon test (pair difference test), p less than 0.083). Advantages and disadvantages of both methods are discussed.