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Related Concept Videos

Antiasthma Drugs: Mast Cell Stabilizers and Anti-IgE Drugs01:25

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Asthma is a chronic respiratory condition for which new therapeutic avenues, including anti-inflammatory drugs like mast cell stabilizers and anti-IgE treatments, continue to be developed.
Mast cell stabilizers, such as cromolyn (also known as sodium cromoglycate) and nedocromil (Tilade), are effective drugs in asthma management. These stabilizers hinder histamine release by skillfully obstructing the activation of mast cells and other cellular entities. Notably, they navigate this task without...
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Related Experiment Video

Updated: Mar 22, 2026

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays
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Clarithromycin Dose-Dependently Stabilizes Rat Peritoneal Mast Cells.

Itsuro Kazama, Kazutomo Saito, Asuka Baba

    Chemotherapy
    |April 19, 2016
    PubMed
    Summary

    Clarithromycin, an antiallergic macrolide, stabilizes mast cells by inhibiting exocytosis. This study provides electrophysiological evidence of clarithromycin

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    Area of Science:

    • Immunology
    • Cell Biology
    • Pharmacology

    Background:

    • Macrolides, including clarithromycin, exhibit antiallergic properties.
    • Mast cell exocytosis, a key allergic response, is monitored via changes in membrane capacitance (Cm).
    • A lack of Cm changes suggests a mast cell-stabilizing effect.

    Purpose of the Study:

    • To investigate the electrophysiological effects of clarithromycin on mast cell exocytosis.
    • To determine if clarithromycin possesses mast cell-stabilizing properties.

    Main Methods:

    • Whole-cell patch clamp technique applied to rat peritoneal mast cells.
    • Measurement of membrane capacitance (Cm) changes during exocytosis.
    • Assessment of plasma membrane deformation using a fluorescent dye.

    Main Results:

    • Clarithromycin significantly inhibited mast cell degranulation.
    • Clarithromycin suppressed GTP-γ-S-induced increases in Cm.
    • The drug reduced plasma membrane deformation during exocytosis.

    Conclusions:

    • Provides the first electrophysiological evidence of clarithromycin's dose-dependent inhibition of exocytosis.
    • Clarithromycin demonstrates mast cell-stabilizing effects.
    • The mechanism involves counteracting plasma membrane deformation during exocytosis.