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Bioprinting three-dimensional cell-laden tissue constructs with controllable degradation.

Zhengjie Wu1, Xin Su1, Yuanyuan Xu1

  • 1Biomanufacturing Engineering Laboratory, Graduate School at Shenzhen, Tsinghua University, Shenzhen, P.R. China.

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|April 20, 2016
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Summary

This study introduces a novel 3D bioprinting method using alginate hydrogels with sodium citrate to control degradation. This technique enhances human corneal epithelial cell proliferation and differentiation for tissue engineering applications.

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Alginate hydrogels are widely used in 3D bioprinting due to their biocompatibility.
  • However, the inability of encapsulated cells to degrade the alginate matrix limits cell proliferation and differentiation.
  • This presents a challenge for creating functional, cell-laden tissue constructs.

Purpose of the Study:

  • To develop a degradation-controllable alginate-based bioink for 3D bioprinting.
  • To improve the proliferation and differentiation of encapsulated human corneal epithelial cells (HCECs).
  • To create advanced cell-laden tissue constructs for tissue engineering.

Main Methods:

  • 3D bioprinting of HCECs within a collagen/gelatin/alginate hydrogel.
  • Incubation with a medium containing sodium citrate to induce controlled degradation.
  • Varying the sodium citrate/sodium alginate mole ratio to tune degradation rates.
  • Assessment of hydrogel structure, cell viability, proliferation, and marker protein expression (CK3).

Main Results:

  • The 3D-printed hydrogel exhibited a stable, interconnected, macroporous structure with over 90% cell viability.
  • Degradation time of the constructs was successfully controlled by adjusting the sodium citrate concentration.
  • Encapsulated HCECs showed enhanced proliferation rates and increased cytokeratin 3 (CK3) expression.
  • The results indicate improved cell behavior compared to non-degradable alginate systems.

Conclusions:

  • The developed sodium citrate-assisted degradation method offers controllable degradation of alginate hydrogels in 3D bioprinting.
  • This approach significantly enhances the proliferation and differentiation of encapsulated HCECs.
  • The findings suggest a promising strategy to improve alginate bioink systems for tissue engineering applications.