Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

5.4K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.4K
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

9.9K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
9.9K
Negative Regulator Molecules01:23

Negative Regulator Molecules

38.8K
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
38.8K
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

3.3K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
3.3K
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

10.3K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
10.3K
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

7.8K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
7.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Distinct genomic and immunologic tumor evolution in germline TP53-driven breast cancers.

Nature communications·2026
Same author

The histone methyltransferase NSD3 oncogene triggers ribosomal DNA transcription, interfering with FOSL2 in cancer.

Cell death & disease·2026
Same author

Protein lactylation: a metabolic signal driving cancer therapy resistance.

Cell death discovery·2026
Same author

The TP53 gene contains a diversity box that makes it more than a tumor suppressor.

Cell death and differentiation·2026
Same author

Gut Microbiota in Lipodystrophies and Obesity: A Common Signature?

Microorganisms·2026
Same author

The role of HECT-type E3 ubiquitin ligases in DNA damage response and repair.

Cell death discovery·2025

Related Experiment Video

Updated: Mar 22, 2026

Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

10.1K

Structural Evolution and Dynamics of the p53 Proteins.

Giovanni Chillemi1, Sebastian Kehrloesser2, Francesca Bernassola3

  • 1CINECA, SCAI-SuperComputing Applications and Innovation Department, Rome 00185, Italy.

Cold Spring Harbor Perspectives in Medicine
|April 20, 2016
PubMed
Summary

The p53 protein family, including p53, p73, and p63, shares common functions in stress response. Molecular dynamics reveal the carboxy-terminal domain regulates DNA binding via an induced-fit mechanism.

More Related Videos

Detection of Aggregation-Prone Behavior in Mutant P53 V157F Breast Cancer Cells Using Multipoint Thioflavin T Fluorescence
04:56

Detection of Aggregation-Prone Behavior in Mutant P53 V157F Breast Cancer Cells Using Multipoint Thioflavin T Fluorescence

Published on: December 30, 2025

315
Purification of Ubiquitinated p53 Proteins from Mammalian Cells
10:55

Purification of Ubiquitinated p53 Proteins from Mammalian Cells

Published on: March 21, 2022

2.9K

Related Experiment Videos

Last Updated: Mar 22, 2026

Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

10.1K
Detection of Aggregation-Prone Behavior in Mutant P53 V157F Breast Cancer Cells Using Multipoint Thioflavin T Fluorescence
04:56

Detection of Aggregation-Prone Behavior in Mutant P53 V157F Breast Cancer Cells Using Multipoint Thioflavin T Fluorescence

Published on: December 30, 2025

315
Purification of Ubiquitinated p53 Proteins from Mammalian Cells
10:55

Purification of Ubiquitinated p53 Proteins from Mammalian Cells

Published on: March 21, 2022

2.9K

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • The p53 protein family, comprising p53, p73, and p63, are critical tumor suppressors.
  • These family members share structural homology and common functions like cell death induction and cell-cycle arrest in response to cellular stress.
  • However, they also exhibit distinct properties influencing their specific roles.

Purpose of the Study:

  • To describe the structural evolution of the p53 family members.
  • To present recent advances in molecular dynamic studies of p53.
  • To elucidate the regulatory role of the carboxy-terminal domain in p53 DNA-binding properties.

Main Methods:

  • Comparative analysis of structural homology within the p53 family.
  • Molecular dynamic simulations of p53.
  • Investigation of carboxy-terminal domain influence on DNA-binding domain (DBD) function.

Main Results:

  • The p53 family members exhibit conserved structural organization and functional similarities.
  • Molecular dynamics studies provide insights into p53's behavior.
  • The carboxy-terminal domain plays a critical role in regulating the DBD, supporting an induced-fit binding mechanism.

Conclusions:

  • The carboxy-terminal domain's regulation of the DBD is crucial for p53's function.
  • p53 binding to promoters is preferentially controlled by the dissociation rate (KOFF) over the association rate (KON) via an induced-fit mechanism.
  • Understanding this mechanism is key to comprehending p53's tumor suppressive activity.