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Updated: Mar 22, 2026

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR
Published on: February 23, 2016
High-field dissolution dynamic nuclear polarization of [1-(13)C]pyruvic acid
Hikari A I Yoshihara1, Emine Can, Magnus Karlsson
1Institute of Physics of Biological Systems, Swiss Federal Institute of Technology, Lausanne, Switzerland. hikari.yoshihara@epfl.ch.
Abstract:
[1-(13)C]pyruvate is the most widely used hyperpolarized metabolic magnetic resonance imaging agent. Using a custom-built 7.0 T polarizer operating at 1.0 K and trityl radical-doped [1-(13)C]pyruvic acid, unextrapolated solution-state (13)C polarization greater than 60% was measured after dissolution and rapid transfer to a spectrometer magnet, demonstrating the signal enhancement attainable using optimized hardware. Slower rates of polarization under these conditions can be largely overcome with higher radical concentrations.
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