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A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells

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Published on: April 11, 2014

ROLE OF ASCORBIC ACID ON TRANSAMINASE ACTIVITIES IN SOME METABOLICALLY ACTIVE TISSUES OF ASPIRIN TREATED RATS.

K K Das¹, S Choudhuri, N M Biswas

¹Department of Physiology, Al-Ameen Medical College, Bijapur - 586 108.

Indian Journal of Physiology and Pharmacology

|April 21, 2016

Summary

This summary is machine-generated.

Aspirin increases liver and kidney enzymes (GOT, GPT) in rats, indicating toxicity. High-dose ascorbic acid supplementation helps restore these enzyme levels, mitigating aspirin

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Area of Science:

Biochemistry
Toxicology
Pharmacology

Background:

Aspirin (acetylsalicylic acid) is a widely used non-steroidal anti-inflammatory drug (NSAID).
Hepatotoxicity and nephrotoxicity are potential adverse effects associated with NSAID use.
Enzymes like aspartate amino transferase (GOT) and alanine amino transferase (GPT) are key biomarkers for liver and kidney function.

Purpose of the Study:

To investigate the effects of aspirin on GOT and GPT activities in plasma, liver, and kidney.
To evaluate the potential protective role of ascorbic acid supplementation against aspirin-induced toxicity.

Main Methods:

Experimental study involving rats treated with aspirin and supplemented with ascorbic acid for seven days.
Assay of GOT and GPT enzyme activities in plasma, liver homogenates, and kidney homogenates.

Main Results:

Aspirin treatment significantly increased plasma GOT and GPT levels while decreasing them in the liver and kidney.
Ascorbic acid supplementation showed no significant changes in plasma and liver enzyme activities.
In the kidney, GOT activity remained unchanged, but GPT activity showed a significant alteration in the ascorbic acid-supplemented group.

Conclusions:

Aspirin exhibits potent hepatotoxic and nephrotoxic effects, as evidenced by altered GOT and GPT levels.
High-dose ascorbic acid supplementation can potentially restore aspirin-induced enzyme alterations in rat liver and kidney, suggesting a protective role.