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A galectin from Eriocheir sinensis functions as pattern recognition receptor enhancing microbe agglutination and
Mengqiang Wang1, Lingling Wang2, Mengmeng Huang1
1Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Rd., Qingdao 266071, Shandong, China.
Fish & Shellfish Immunology
|April 21, 2016
Summary
Chinese mitten crab galectin (EsGal) is a key immune protein. It recognizes and eliminates pathogens, enhancing the crab's innate immunity against microbial infections.
Area of Science:
- Immunology
- Marine Biology
- Biochemistry
Background:
- Galectins are pattern recognition receptors (PRRs) crucial for innate immunity in diverse species.
- Understanding crustacean galectins is vital for aquaculture and disease resistance.
Purpose of the Study:
- To clone and characterize the Chinese mitten crab Eriocheir sinensis galectin (EsGal).
- To investigate the immune functions of EsGal in response to microbial challenges.
Main Methods:
- Cloning of EsGal cDNA using rapid amplification of cDNA ends (RACE) and expressed sequence tags (ESTs).
- Analysis of EsGal protein structure and tissue expression patterns.
- Recombinant EsGal (rEsGal) production and functional assays, including PAMP binding, agglutination, and in vitro encapsulation.
Main Results:
- EsGal cDNA encodes a 218-amino acid protein with conserved galectin domains.
- EsGal mRNA is constitutively expressed and upregulated upon microbial stimulation.
- rEsGal binds PAMPs, agglutinates various microbes, and enhances hemocyte encapsulation, demonstrating broad antimicrobial activity.
Conclusions:
- EsGal plays a critical role in the innate immune response of Chinese mitten crabs.
- EsGal contributes to pathogen recognition and elimination, offering potential for disease management in aquaculture.
Keywords:
Eriocheir sinensisGalectinHaemocytes encapsulationInnate immunityMicrobes agglutinationPAMPs binding activityPattern recognition receptorMore Related Videos
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