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Updated: Mar 22, 2026
Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells
Published on: November 28, 2015
Cytoplasmic polyadenylation element-binding protein 4 is highly expressed in human glioma
Hongchao Hu1, Lulu Cong, Chaohui Liang
1aDepartment of Neurosurgery, The Second Hospital of Hebei Medical University, Hebei bDepartment of Neurosurgery, Handan Central Hospital, Handan, China.
Abstract:
Cytoplasmic polyadenylation element-binding protein 4 (CPEB4) is a highly conserved, sequence-specific RNA-binding protein that recruits translational repression or cytoplasmic polyadenylation machinery to target mRNAs. Recent studies have shown that CPEBs are expressed in somatic tissues and have essential functions supporting tumor growth, vascularization, and invasion. Overexpression of CPEB4 has been reported in pancreatic ductal adenocarcinoma and is associated with poor prognoses. However, whether CPEB4 plays a role in the tumorigenesis of gliomas is unknown. Here, we analyzed the expression of CPEB4 in gliomas. The expression profiles of CPEB4 mRNA and protein in nine normal brain tissues and 63 gliomas were detected using immunohistochemistry, real-time PCR, and western blotting. CPEB4-positive expression was significantly correlated with the pathological grade of glioma; abundant expression was observed in high-grade gliomas, whereas little or no expression was observed in normal astrocytes. Immunohistochemistry staining indicated that CPEB4 was mainly localized in the cytoplasm. In addition, CPEB4 was more highly expressed in U87 glioma cells than in U251 cells. CPEB4 expression significantly correlated with the grade in clinical gliomas. This study suggested that CPEB4 might play a role in the pathogenesis of glioma.
Insights
Cytoplasmic polyadenylation element-binding protein 4 (CPEB4) is overexpressed in gliomas and correlates with higher pathological grades. This suggests CPEB4 may contribute to glioma development and progression.
Area of Science:
- Neuro-oncology
- Molecular Biology
- Cancer Research
Background:
- Cytoplasmic polyadenylation element-binding proteins (CPEBs) are involved in tumor growth and vascularization.
- CPEB4 overexpression is linked to poor prognoses in pancreatic cancer.
- The role of CPEB4 in glioma tumorigenesis remains unexplored.
Purpose of the Study:
- To investigate the expression and potential role of CPEB4 in glioma pathogenesis.
- To analyze the correlation between CPEB4 expression and glioma pathological grade.
Main Methods:
- Analysis of CPEB4 mRNA and protein expression in normal brain tissues and gliomas.
- Utilized immunohistochemistry, real-time PCR, and western blotting.
- Compared CPEB4 expression in U87 and U251 glioma cell lines.
Main Results:
- CPEB4 expression was significantly higher in gliomas compared to normal brain tissues.
- CPEB4 expression positively correlated with advanced pathological grades of glioma.
- CPEB4 was predominantly localized in the cytoplasm and showed differential expression in glioma cell lines.
Conclusions:
- CPEB4 is upregulated in gliomas and associated with tumor grade.
- CPEB4 may play a role in the development and progression of gliomas.
- Further research into CPEB4's function in glioma is warranted.
