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Microbial Biosensors

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

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Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

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Simultaneous detection of E. coli K12 and S. aureus Using a Continuous Flow Multijunction Biosensor.

Inae Lee¹, Soojin Jun²

¹Author Lee is with Dept. of Molecular Biosciences and Bioengineering, Univ. of Hawaii, Honolulu, Hawaii, 96822, U.S.A.

Journal of Food Science

|April 21, 2016

Summary

This study developed a continuous flow multijunction biosensor for rapid, simultaneous detection of foodborne pathogens like Escherichia coli K12 and Staphylococcus aureus. The novel biosensor enables real-time monitoring, crucial for food safety and preventing outbreaks.

Keywords:

carbon nanotubes multiplexing pathogens rapid detection

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Area of Science:

Biosensor technology
Food safety
Microbiology

Background:

Foodborne illnesses pose significant risks to public health.
Rapid and continuous monitoring of foodborne pathogens is essential for food manufacturers.
Existing methods often lack real-time detection capabilities.

Purpose of the Study:

To fabricate and characterize a continuous flow multijunction biosensor.
To enable simultaneous detection of Escherichia coli K12 and Staphylococcus aureus.
To assess the sensor's performance for real-time food pathogen monitoring.

Main Methods:

Fabrication of junction biosensors using gold-plated tungsten wires, polyethylenimine, and single-walled carbon nanotubes.
Functionalization of biosensor junctions with streptavidin and specific biotinylated antibodies for E. coli K12 and S. aureus.
Continuous flow system utilizing a fluidic channel for sample introduction and electric current change measurement.

Main Results:

The continuous flow multijunction biosensor demonstrated enhanced electric signal responses compared to stationary sensors.
Linear regression was observed for both E. coli and S. aureus detection within the range of 10(2) to 10(5) CFU/mL.
Multiplexed detection of E. coli K12 and S. aureus at levels as low as 10(2) CFU/mL was achieved within 2 minutes.

Conclusions:

The developed continuous flow multijunction biosensor is effective for rapid and simultaneous detection of foodborne pathogens.
This technology shows significant potential for real-time, continuous monitoring in food safety applications.
The biosensor facilitates early detection, aiding in the prevention of foodborne illness outbreaks.