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Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

Alternative RNA Splicing

Alternative RNA Splicing

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

RNA Splicing

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Gene Regulation During Sporulation

Gene Regulation During Sporulation

Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...

Regulation of Expression at Multiple Steps

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The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...

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Related Experiment Video

Updated: Mar 22, 2026

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

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Published on: June 30, 2022

Splicing-Mediated Autoregulation Modulates Rpl22p Expression in Saccharomyces cerevisiae.

Jason Gabunilas¹, Guillaume Chanfreau^1,2

¹Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California, United States of America.

|April 21, 2016

Summary

This summary is machine-generated.

Rpl22p protein inhibits splicing of its own pre-mRNA (RPL22B) through a stem-loop structure. This autoregulatory mechanism fine-tunes Rpl22p levels based on cellular ribosome biogenesis needs.

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Published on: September 17, 2016

Area of Science:

Molecular Biology
Gene Regulation
Yeast Genetics

Background:

Ribosomal protein genes (RPGs) are highly expressed in Saccharomyces cerevisiae and tightly regulated.
Precise gene-by-gene regulation of RPG pre-mRNA splicing remains incompletely understood.

Purpose of the Study:

To investigate the regulatory mechanisms of ribosomal protein pre-mRNA splicing.
To elucidate the extraribosomal functions of Rpl22p in gene expression regulation.

Main Methods:

In vivo and in vitro splicing assays.
Analysis of pre-mRNA secondary structures.
Assessment of U1 snRNP recruitment.

Main Results:

Rpl22p inhibits splicing of RPL22B pre-mRNA via a stem-loop structure in the intron.
This inhibition involves reduced co-transcriptional U1 snRNP recruitment.
RPL22B splicing inhibition down-regulates mature transcript levels under stress and during suppressed ribosome biogenesis.

Conclusions:

Rpl22p exhibits an autoregulatory role in fine-tuning its own expression.
This mechanism adjusts Rpl22p levels and paralog composition according to ribosome biogenesis demands.