Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A monolithic patch-clamping amplifier with capacitive feedback.

J Prakash1, J J Paulos, D N Jensen

  • 1Department of Electrical and Computer Engineering, North Carolina State University, Raleigh 27695-7911.

Journal of Neuroscience Methods
|March 1, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

DB-SegNet: optimized framework for glaucoma detection and optic structure segmentation from retinal fundus images.

Scientific reports·2025
Same author

Corrigendum to "Development of bioactive chitosan patch with ciprofloxacin loaded TiO₂ nanoparticles for wound healing application" [Int. J. Biol. Macromol. 31 (2025) 146302].

International journal of biological macromolecules·2025
Same author

Development of bioactive chitosan patch with ciprofloxacin loaded TiO₂ nanoparticles for wound healing application.

International journal of biological macromolecules·2025
Same author

Retraction Note: A vehicular network based intelligent transport system for smart cities using machine learning algorithms.

Scientific reports·2024
Same author

Surface modification of extracellular vesicles with polyoxazolines to enhance their plasma stability and tumor accumulation.

Biomaterials·2024
Same author

Corrigendum to "Numerical simulation of heat transfer in blood flow altered by electroosmosis through tapered micro-vessels" [Microvasc. Res. 118 (2018) 162-172].

Microvascular research·2024
Same journal

Detection of cochlear microphonic for differential diagnosis between auditory neuropathy mice and noise-induced sensorineural hearing loss mice.

Journal of neuroscience methods·2026
Same journal

Assessment metrics for pain control in rats: A methodological commentary.

Journal of neuroscience methods·2026
Same journal

Infant EEG preprocessing pipelines: A capability framework and current gaps in practice.

Journal of neuroscience methods·2026
Same journal

Methods for measuring neural activity during voluntary wheel running.

Journal of neuroscience methods·2026
Same journal

Serotype-dependent differences in AAV cellular transduction rates in the hypothalamus of Arctic ground squirrels.

Journal of neuroscience methods·2026
Same journal

Rapid generation of human sensory neurons from iPSC for modeling of peripheral neuropathies.

Journal of neuroscience methods·2026
See all related articles

This study introduces a novel capacitive patch-clamp technique, overcoming limitations of traditional resistive methods for measuring ion channel activity. The new approach offers competitive performance for cellular membrane research.

Area of Science:

  • Biophysics
  • Electrophysiology
  • Neuroscience

Background:

  • Patch-clamping is a crucial technique for studying ion transport across cellular membranes.
  • Conventional resistive patch-clamp amplifiers face bandwidth and saturation limitations.
  • Observing individual ion channel molecule transitions requires high-resolution measurement.

Purpose of the Study:

  • To present an alternative patch-clamping technique utilizing a capacitor as the transimpedance element.
  • To overcome bandwidth and saturation limitations inherent in resistive patch-clamp amplifiers.
  • To demonstrate a monolithic CMOS implementation of the capacitive patch-clamp system.

Main Methods:

  • Fabrication of a monolithic integrated circuit using 5-micron CMOS technology.

Related Experiment Videos

  • Inclusion of an on-chip operational amplifier, a capacitor array (0.03 pF to 30 pF), and associated switches.
  • Utilizing a capacitor as the transimpedance element instead of a resistor.

    • Main Results:

    • The developed system achieves performance competitive with conventional instruments up to a 10 kHz bandwidth.
    • Voltage noise from the CMOS operational amplifier was identified as the primary limitation on overall noise performance.
    • Effective performance was demonstrated for small input capacitances (≤ 5 pF).

    Conclusions:

    • The capacitive patch-clamp technique offers a viable alternative to resistive methods, mitigating bandwidth and saturation issues.
    • CMOS technology enables the fabrication of compact, integrated patch-clamp systems.
    • Further optimization of amplifier noise is necessary for enhanced performance across a wider range of capacitances.