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Related Experiment Video

Updated: Mar 22, 2026

Assessment of Mitochondrial Oxygen Consumption Using a Plate Reader-based Fluorescent Assay
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Assessment of Mitochondrial Oxygen Consumption Using a Plate Reader-based Fluorescent Assay

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Eating increases oxidative damage in a reptile.

Michael W Butler1, Thomas J Lutz2, H Bobby Fokidis3

  • 1Department of Biology, Lafayette College, Easton, PA 18042, USA butlermw@lafayette.edu.

The Journal of Experimental Biology
|April 22, 2016
PubMed
Summary

Eating imposes a significant oxidative cost, increasing reactive oxygen metabolites (ROMs) by 155% in corn snakes. This highlights a major physiological burden of digestion previously overlooked in ecological studies.

Keywords:
DigestionFood intakeHydroperoxidesPrandial stateReactive oxygen metabolitesSpecific dynamic action

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Area of Science:

  • Physiology
  • Zoology
  • Biochemistry

Background:

  • Digestion provides nutrients and energy but incurs physiological costs.
  • While energy expenditure is well-studied, other physiological costs of eating are less explored.
  • Oxidative stress, an imbalance between pro-oxidants and antioxidants, is a potential cost of metabolic processes.

Purpose of the Study:

  • To investigate the impact of eating on systemic pro-oxidant (reactive oxygen metabolite, ROM) levels and antioxidant capacity.
  • To quantify the oxidative cost of meal processing in corn snakes (Pantherophis guttatus).

Main Methods:

  • Plasma samples were collected from corn snakes during absorptive (post-meal) and non-absorptive (baseline) states.
  • Systemic reactive oxygen metabolite (ROM) levels and antioxidant capacity were measured.
  • Data were analyzed controlling for circulating nutrient levels.

Main Results:

  • A minimal increase (4%) in antioxidant capacity was observed during the absorptive state compared to baseline.
  • A substantial increase (nearly 155%) in ROM levels was detected during the absorptive state, even after controlling for nutrient levels.
  • The oxidative cost of eating was found to be significantly higher than costs associated with long-distance flight or immune responses in other species.

Conclusions:

  • Eating incurs a significant oxidative cost in corn snakes, characterized by a marked increase in ROMs.
  • This finding underscores the importance of investigating non-energetic physiological costs of meal processing.
  • Further research is needed to elucidate the mechanisms driving eating-induced ROM increases and their taxonomic prevalence.