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Related Experiment Video

Updated: Mar 22, 2026

A Technique to Functionalize and Self-assemble Macroscopic Nanoparticle-ligand Monolayer Films onto Template-free Substrates
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Nanocoating for biomolecule delivery using layer-by-layer self-assembly.

M Keeney1, X Y Jiang1, M Yamane2

  • 1Department of Orthopaedic Surgery, 300 Pasteur Dr., Edwards R105, Stanford, CA 94305, USA.

Journal of Materials Chemistry. B
|April 22, 2016
PubMed
Summary
This summary is machine-generated.

Layer-by-layer (LbL) self-assembly offers versatile biomolecule delivery for applications in medicine and technology. This review explores LbL methods for controlled release and discusses challenges to clinical translation.

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Area of Science:

  • Materials Science
  • Biotechnology
  • Nanotechnology

Background:

  • Layer-by-layer (LbL) self-assembly is a widely adopted technique for fabricating films in diverse fields.
  • Its advantages include ease of manufacture, low cost, mild conditions, precise thickness control, and material versatility.
  • LbL technology has shown significant promise for biomolecule delivery, yet clinical translation remains slow.

Purpose of the Study:

  • To review methods and mechanisms for loading biomolecules into LbL films.
  • To highlight recent advancements in LbL coatings for delivering various biomolecules (proteins, DNA, particles, viruses).
  • To discuss strategies for co-delivery and compartmentalization of multiple biomolecules.

Main Methods:

  • Overview of LbL self-assembly principles for film fabrication.
  • Analysis of biomolecule loading and controlled release mechanisms within LbL structures.
  • Review of recent literature on LbL applications for diverse biomolecule delivery systems.

Main Results:

  • LbL films can effectively encapsulate and control the release of proteins, polypeptides, DNA, particles, and viruses.
  • Compartmentalization strategies enable co-delivery of multiple biomolecules with temporal control.
  • Significant progress has been made in tailoring LbL coatings for specific biomolecule delivery needs.

Conclusions:

  • LbL self-assembly is a powerful platform for advanced biomolecule delivery systems.
  • Overcoming obstacles to clinical translation is crucial for realizing the full potential of LbL technologies.
  • Future directions involve developing enabling technologies for broader clinical adoption.