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Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...

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Updated: Mar 22, 2026

Analysis of Translation in the Developing Mouse Brain using Polysome Profiling

Analysis of Translation in the Developing Mouse Brain using Polysome Profiling

Published on: May 22, 2021

Cell type-specific transcriptome profiling in mammalian brains.

Peter R LoVerso¹, Feng Cui²

¹Thomas H. Gosnell School of Life Sciences, Rochester Institute of Technology, One Lomb Memorial Dr., Rochester, NY 14623.

Frontiers in Bioscience (Landmark Edition)

|April 22, 2016

Summary

This summary is machine-generated.

Neuroscience advances enable cell-specific gene discovery. These molecular signatures from transcriptomic studies help classify neural cells and subtypes, aiding neurological disease research.

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Last Updated: Mar 22, 2026

Analysis of Translation in the Developing Mouse Brain using Polysome Profiling

Analysis of Translation in the Developing Mouse Brain using Polysome Profiling

Published on: May 22, 2021

Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling |

Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling |

Published on: November 13, 2011

Cell Type-specific Gene Expression Profiling in the Mouse Liver

Cell Type-specific Gene Expression Profiling in the Mouse Liver

Published on: September 17, 2019

Area of Science:

Neuroscience
Genomics
Molecular Biology

Background:

Mammalian brains exhibit diverse cell types.
High-throughput technologies like microarrays and next-generation sequencing (NGS) provide genomic-scale gene expression data.
Novel genes critical for cell type-specific transcriptional regulation have been identified.

Purpose of the Study:

To describe experimental techniques for purifying neural cells.
To review transcriptomic studies of common neural cell types using microarrays or NGS.
To discuss future prospects in cell type-specific research.

Main Methods:

Neural cell purification techniques.
Analysis of gene expression data from microarrays and next-generation sequencing (NGS).
Identification of differentially expressed genes as molecular signatures.

Main Results:

Identification of novel genes critical for cell type-specific transcriptional regulation.
Development of gene expression-based molecular signatures for neural cell classification.
Potential for differentiating neural cell subtypes and linking them to neurological diseases.

Conclusions:

Gene expression profiling is a powerful tool for neural cell characterization.
Molecular signatures derived from transcriptomic data enhance our understanding of neural cell diversity.
Cell type-specific research holds promise for advancing neurological disease diagnostics and therapeutics.