Related Experiment Video
Updated: Mar 22, 2026

10:34
FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors
Published on: August 20, 2012
23.9K
Overcoming limitations of FRET measurements
Silas J Leavesley1,2,3, Thomas C Rich2,3,4
1Department of Chemical and Biomolecular Engineering, University of South Alabama, Mobile, Alabama.
Abstract
No abstract available in PubMed .
Keywords:
FLIMFRETFörster resonance energy transferfluorescenceimaginglifetimemicroscopyspectral imagingspectroscopyMore Related Videos
Related Concept Videos
Problem-Solving: Tuning of a Guitar String
1.2K
In the case of stringed instruments like the guitar, the elastic property that determines the speed of the sound produced is its linear mass density or the mass per unit length. This is simply called the linear density. If the string's linear density is constant along the string, then the linear density is simply the total mass divided by the total length.
The string's wave speed can be regulated by varying the linear density. Tension is the other property that determines the speed of...
The string's wave speed can be regulated by varying the linear density. Tension is the other property that determines the speed of...
1.2K
Design Example: Measuring Distance Between Two Points with Obstructions
500
When measuring distances in areas with physical obstructions, such as a lake in a field, surveyors must employ techniques to calculate accurate lengths without direct line measurements. One effective method is the offset technique, which allows for precise distance estimation over inaccessible stretches.In this scenario, a surveyor must measure a side of an area that crosses a lake. Since the measuring tape cannot span the lake, the surveyor begins by establishing a baseline that aligns with...
500
Design Example: Strain Gauge Bridge or Wheatstone Bridge
1.2K
The utilization of strain gauges as transducers for converting mechanical strain into electrical signals is a common practice in various engineering applications. These strain gauges are frequently integrated into Wheatstone bridge circuits to accurately measure parameters such as force or pressure. Within this context, each element within the circuit exhibits a resistance that undergoes subtle variations when subjected to mechanical strain. The primary objective is to convert minuscule...
1.2K
Uncertainty in Measurement: Reading Instruments
55.6K
Counting is the type of measurement that is free from uncertainty, provided the number of objects being counted does not change during the process. Such measurements result in exact numbers. By counting the eggs in a carton, for instance, one can determine exactly how many eggs are there in the carton. Similarly, the numbers of defined quantities are also exact. For example, 1 foot is exactly 12 inches, 1 inch is exactly 2.54 centimeters, and 1 gram is exactly 0.001 kilograms. Quantities...
55.6K
Rules for Significant Figures
41.7K
In any measurement, the precision of the measuring tool is an essential factor. An ordinary ruler, for example, can measure length to the closest millimeter; a caliper, on the other hand, can measure length to the nearest 0.01 mm. As a result, the caliper is a more precise measurement tool because it can measure extremely minute changes in length. The measurements will be more accurate if the measuring tool is more precise.
It should be emphasized that when we represent measured values, the...
It should be emphasized that when we represent measured values, the...
41.7K
Common Leveling Mistakes and Errors
579
A survey team is tasked with determining the elevation difference between points Point A and Point B, separated by uneven terrain. They use a leveling instrument and a leveling rod.Common MistakesMisreading the Rod: During a backsight reading at Point A, the instrumentman observes the rod partially obscured by tall grass. Instead of reading 1.135 m, they mistakenly record 1.735 m due to the misalignment of the crosshair with the wrong graduation. This error adds 0.600 m to all subsequent...
579

