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Imaging Biological Samples with Optical Microscopy

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Related Experiment Video

Updated: Mar 22, 2026

Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures

Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures

Published on: December 1, 2023

Snapshot Hyperspectral Volumetric Microscopy.

Jiamin Wu¹, Bo Xiong¹, Xing Lin¹

¹Department of Automation, Tsinghua National Laboratory for Information Science and Technology (TNList), Tsinghua University, Beijing, 100084, China.

Scientific Reports

|April 23, 2016

Summary

This summary is machine-generated.

This study introduces snapshot hyperspectral volumetric microscopy for high-resolution 3D imaging. The novel method enables video-rate acquisition and analysis of complex biological specimens, advancing dynamic imaging capabilities.

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Area of Science:

Biomedical Imaging
Microscopy
Spectroscopy

Background:

High-dimensional biological specimen analysis requires capturing spatial, temporal, and spectral data.
Existing methods face challenges in data throughput and multiplexing for complex imaging.

Purpose of the Study:

To develop snapshot hyperspectral volumetric microscopy for high-resolution 3D imaging at video rate.
To address limitations in data throughput and multiplexing for dynamic biological specimens.

Main Methods:

Utilized a novel four-dimensional (4D) deconvolution algorithm for computational reconstruction.
Validated the approach with numerical simulations and experimental results on a prototype system.

Main Results:

Achieved high-resolution hyperspectral volume reconstruction (~1000μm × 1000μm × 500μm) at video rate.
Demonstrated applications in bright-field component analysis and multi-fluorescence spectral unmixing.
Successfully imaged moving fluorescent beads and GFP-labeled Drosophila larvae.

Conclusions:

The developed hyperspectral volumetric microscopy offers a powerful tool for dynamic biological imaging.
The method shows great potential for observing multiple fluorescent markers in live specimens.
Advances in computational imaging enable comprehensive analysis of complex biological samples.