Pair interactions among ternary DPPC/POPC/cholesterol mixtures in liquid-ordered and liquid-disordered phases
Jing Yang1, Jordi Martí1, Carles Calero2
1Department of Physics, Technical University of Catalonia-Barcelona Tech, B4-B5 Northern Campus, Jordi Girona 1-3, 08034 Barcelona, Catalonia, Spain. jing.yang@upc.edu jordi.marti@upc.edu.
Soft Matter
|April 23, 2016
Summary
Cell membrane components interact differently based on saturation. Cholesterol prefers saturated lipids, while lipids favor interactions with similar saturation levels, impacting membrane packing.
Area of Science:
- Membrane biophysics
- Computational biology
- Biochemistry
Background:
- Phospholipids and cholesterol are vital cell membrane constituents.
- Understanding their interactions is crucial for membrane function.
- Lipid saturation influences membrane phase behavior.
Purpose of the Study:
- To investigate lipid-lipid and cholesterol-lipid interactions in ternary mixtures.
- To analyze these interactions across liquid-ordered and liquid-disordered membrane phases.
- To elucidate the role of lipid saturation in membrane component interactions.
Main Methods:
- Microsecond molecular dynamics simulations.
- Ternary mixtures of dipalmitoylphosphatidylcholine (DPPC)/1-palmitoyl-2-oleoylphosphatidylcholine (POPC)/cholesterol.
- Analysis of lipid packing and interaction preferences.
Main Results:
- A competition exists between cholesterol-lipid packing and lipid-lipid packing during phase transitions.
- Lipid-lipid interaction preference follows: saturated-saturated > monounsaturated-monounsaturated > saturated-monounsaturated.
- Cholesterol-saturated lipid interactions are more favorable than cholesterol-monounsaturated lipid interactions.
- Observed interactions align with experimental push-pull force data.
Conclusions:
- Lipid saturation significantly dictates interaction preferences within cell membranes.
- Cholesterol's interaction with saturated lipids influences membrane organization.
- Simulation results provide insights into the complex interplay of membrane components.
More Related Videos
10:08Phase Behavior of Charged Vesicles Under Symmetric and Asymmetric Solution Conditions Monitored with Fluorescence Microscopy
Published on: October 24, 2017
9.7K
12:18Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions
Published on: August 3, 2021
4.2K
Related Concept Videos
01:28Phase Diagrams of Ternary Systems
52
Consider a ternary system, which is composed of three components: water (W), ethanoic acid (E), and trichloromethane (T). Here, Ethanoic acid (E) is fully miscible with both water (W) and trichloromethane (T), meaning it can mix entirely with either of them. However, water and trichloromethane have partial miscibility, meaning they can only mix to a certain extent, beyond which two separate phases will form.The phase diagram of a ternary system is represented as an equilateral triangle, where...
52
01:29The Colloidal State
97
The formation of a colloidal system is exemplified by an aqueous solution containing Cl− ions is introduced to another containing Ag+ ions, resulting in the precipitation of solid AgCl as extremely tiny crystals. Instead of settling out as a filterable precipitate, these crystals remain suspended in the liquid, showcasing a colloidal system.A colloidal system involves colloidal particles within the approximate range of 1 to 1000 nm in at least one dimension, dispersed in a medium called...
97
01:30Micelles
157
Micelle formation is an intricate process that hinges on the properties of amphiphilic or amphipathic molecules and the conditions of the system in which they are found. Amphiphilic molecules, which have both hydrophilic (water-attracting) and hydrophobic (water-repelling) parts, play a critical role in this process.In aqueous environments, these molecules arrange themselves such that their hydrophilic heads are turned towards the water phase, while their hydrophobic tails are oriented away...
157
01:26Membrane Fluidity
17.7K
Membrane fluidity is explained by the fluid mosaic model of the cell membrane, which describes the plasma membrane structure as a mosaic of components—including phospholipids, cholesterol, proteins, and carbohydrates—that gives the membrane a fluid character.
Mosaic nature of the membrane
The mosaic characteristic of the membrane helps the plasma membrane remain fluid. The integral proteins and lipids exist as separate but loosely-attached molecules in the membrane. The membrane is...
Mosaic nature of the membrane
The mosaic characteristic of the membrane helps the plasma membrane remain fluid. The integral proteins and lipids exist as separate but loosely-attached molecules in the membrane. The membrane is...
17.7K
01:23Membrane Fluidity
178.8K
Cell membranes are composed of phospholipids, proteins, and carbohydrates loosely attached to one another through chemical interactions. Molecules are generally able to move about in the plane of the membrane, giving the membrane its flexible nature called fluidity. Two other features of the membrane contribute to membrane fluidity: the chemical structure of the phospholipids and the presence of cholesterol in the membrane.
178.8K
02:56Intermolecular Forces and Physical Properties
29.7K
29.7K