Related Experiment Videos
Modulation of catecholamine metabolism in synaptosomes by a neuroregulatory factor from mammalian brain
A Pastuszko1, D K Yee, D F Wilson
1Department of Biochemistry and Biophysics, Medical School, University of Pennsylvania, Philadelphia 19104.
Abstract:
Incubation of synaptosomes from rat brain with bovine brain extract caused inhibition of oxidative deamination of dopamine, decreased formation of 3,4-dihydroxyphenylacetic acid, increased formation of norepinephrine and its N-methyl derivatives and increased release of catecholamines. Omission of Ca2+ from the extrasynaptosomal medium completely blocked the brain extract induced release of [3H]catecholamines and decreased, by about 80%, the effect on changes in catecholamine metabolism. Chromatography of the brain extract on Sephadex-G25 columns resulted in an active compound eluting at the position expected for compounds with molecular weights in the region of 1500 to 2500 Da.