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A virulent phage JHP against Pseudomonas aeruginosa showed infectivity against multiple genera

Komal Ameer Khawaja1, Mahd Rauf1, Zaigham Abbas1

  • 1Department of Microbiology and Molecular Genetics, University of the Punjab, Quaid-e-Azam Campus, Lahore, Pakistan.

Journal of Basic Microbiology
|April 24, 2016
PubMed

Insights

Phage therapy offers a promising alternative to antibiotics against resistant bacteria. A novel Siphoviridae bacteriophage, JHP, shows broad-spectrum activity against pathogens like Pseudomonas aeruginosa and Enterobacteriaceae.

Area of Science:

  • Microbiology
  • Virology
  • Infectious Diseases

Background:

  • Antibiotic resistance in bacteria is a critical global health issue.
  • Bacteriophage therapy presents a viable alternative to combat multidrug-resistant pathogens.
  • Novel bacteriophages require characterization for therapeutic potential.

Purpose of the Study:

  • To evaluate the host range and characteristics of a novel bacteriophage, JHP.
  • To assess the suitability of bacteriophage JHP for phage therapy applications.

Main Methods:

  • Assessed bacteriophage JHP infectivity against various bacterial genera, including multidrug-resistant clinical isolates.
  • Determined optimal conditions (pH, temperature) for antibacterial activity and storage.
  • Characterized JHP's lytic properties, genome size, and morphology (Siphoviridae family).

Main Results:

  • Bacteriophage JHP exhibited broad-spectrum infectivity against Pseudomonas aeruginosa, Enterobacteriaceae, and other human pathogens.
  • Optimal activity occurred at pH 7 and 37°C; stable storage at 4°C or -20°C.
  • JHP has a latent period of 25 min, burst size of 433 virions/cell, a ~30 kb genome, and Siphoviridae morphology (115 nm head, 152 nm tail).

Conclusions:

  • The broad-spectrum Siphoviridae bacteriophage JHP is a promising candidate for phage therapy.
  • JHP demonstrates significant potential in combating antibiotic-resistant bacterial infections.
Keywords:
BacteriophagesJHPPhage therapyPseudomonas aeruginosaSiphoviridae

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