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An albumin nanocomplex-based endosomal pH-activatable on/off probe system.

Changkyu Lee1, Seunghyun Lee1, Le Quang Thao1

  • 1School of Pharmacy, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon, Gyeonggi-do 16419, Republic of Korea.

Colloids and Surfaces. B, Biointerfaces
|April 25, 2016
PubMed
Summary

This study introduces a novel pH-sensitive albumin nanocomplex that self-assembles at neutral pH and disassembles in acidic tumor environments. This smart system releases zinc and emits fluorescence, enabling targeted cancer cell destruction and imaging.

Keywords:
AlbuminNanocomplexSelf-assemblyTumor targetingZinc-imidazole interactionpH-sensitivity

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Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Cancer Theranostics

Background:

  • Albumin is a biocompatible material for nanoparticle fabrication.
  • pH-sensitive drug delivery systems are crucial for targeted cancer therapy.

Purpose of the Study:

  • To develop a pH-sensitive albumin nanocomplex for tumor-targeted drug delivery and imaging.
  • To investigate the theranostic potential of zinc-imidazole coordinated albumin nanocomplexes.

Main Methods:

  • Albumin conjugation with imidazole and PEG derivatives.
  • Self-assembly of albumin nanocomplexes (C/BQ-PBI Zn NCs) around pH 7.4.
  • Evaluation of pH-dependent disassembly, fluorescence, and Zn(2+) release.
  • Assessment of cytotoxicity in breast cancer (MCF-7) and lung adenocarcinoma (A549) cells.
  • Confocal laser scanning microscopy for cellular uptake and localization.

Main Results:

  • Albumin nanocomplexes (∼116nm) self-assembled at pH 7.4 and disassembled at pH 5.0.
  • pH-sensitive fluorescence "on/off" switching via FRET mechanism.
  • Significant cytotoxicity observed in cancer cells due to increased intracellular Zn(2+).
  • Strong fluorescence signals detected in the endosomal region of MCF-7 cells.

Conclusions:

  • The developed albumin nanocomplex exhibits excellent pH-sensitivity and biocompatibility.
  • The system acts as an effective tumor-targeting probe and drug carrier.
  • This albumin nanocomplex holds promise for theranostic applications in cancer treatment.