Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of Cell Death01:30

Overview of Cell Death

11.0K
Cell death is an essential process where the body gets rid of old or damaged cells. Cell proliferation and death need to be balanced, as an imbalance between the two may lead to cancer or autoimmune diseases.
Cell death was observed in the early 19th century, but there was no experimental evidence to prove it. In 1842, Carl Vogt first discovered cell death in a metamorphic toad; however, it was not termed ‘cell death.’ Scientists discovered different cell death pathways only in the...
11.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Philosophical foundations of plant awareness for sustainability.

Trends in plant science·2026
Same author

AquIRE reveals the mechanisms of clinically induced RNA damage and the conservation and dynamics of glycoRNAs.

Nucleic acids research·2026
Same author

Leaf as nursery: insights on hormonal control of asexual reproduction from Kalanchoë pinnata.

Journal of experimental botany·2025
Same author

Rose Bengal Is a Precise Pharmacological Tool Triggering Chloroplast-Driven Programmed Cell Death in Plants, Dependent on Calcium and Mitochondria, and Associated With Early Transcriptional Reprogramming.

Plant direct·2025
Same author

FT-like genes in Cannabis and hops: sex specific expression and copy-number variation may explain flowering time variation.

BMC genomics·2025
Same author

Transcriptional signatures associated with waterlogging stress responses and aerenchyma formation in barley root tissue.

Annals of botany·2025

Related Experiment Video

Updated: Mar 22, 2026

Author Spotlight: A Streamlined Approach to Studying Cell Death Initiation in Hypersensitive Response
06:06

Author Spotlight: A Streamlined Approach to Studying Cell Death Initiation in Hypersensitive Response

Published on: November 10, 2023

2.1K

Methods to Study Plant Programmed Cell Death.

Joanna Kacprzyk1, Adrian N Dauphinee2, Patrick Gallois3

  • 1School of Biology and Environmental Science, University College Dublin, Dublin 4, Ireland.

Methods in Molecular Biology (Clifton, N.J.)
|April 25, 2016
PubMed
Summary
This summary is machine-generated.

This chapter details methods for studying programmed cell death (PCD) in plants, crucial for development and stress response. Techniques like root hair and electrolyte leakage assays, along with the lace plant model, enable quantitative PCD analysis.

Keywords:
Abiotic stressDevelopmental programmed cell deathElectrolyte leakageLeaf morphogenesisProgrammed cell deathRoot hair assay

More Related Videos

A β-glucuronidase GUS Based Cell Death Assay
07:35

A β-glucuronidase GUS Based Cell Death Assay

Published on: May 6, 2011

28.8K
Assay for Pathogen-Associated Molecular Pattern PAMP-Triggered Immunity PTI in Plants
08:45

Assay for Pathogen-Associated Molecular Pattern PAMP-Triggered Immunity PTI in Plants

Published on: September 9, 2009

28.1K

Related Experiment Videos

Last Updated: Mar 22, 2026

Author Spotlight: A Streamlined Approach to Studying Cell Death Initiation in Hypersensitive Response
06:06

Author Spotlight: A Streamlined Approach to Studying Cell Death Initiation in Hypersensitive Response

Published on: November 10, 2023

2.1K
A β-glucuronidase GUS Based Cell Death Assay
07:35

A β-glucuronidase GUS Based Cell Death Assay

Published on: May 6, 2011

28.8K
Assay for Pathogen-Associated Molecular Pattern PAMP-Triggered Immunity PTI in Plants
08:45

Assay for Pathogen-Associated Molecular Pattern PAMP-Triggered Immunity PTI in Plants

Published on: September 9, 2009

28.1K

Area of Science:

  • Plant Biology
  • Cellular Biology
  • Molecular Biology

Background:

  • Programmed cell death (PCD) is essential for plant development, pathogen defense, and stress responses.
  • Understanding the mechanisms of PCD is critical for plant science and agriculture.

Purpose of the Study:

  • To provide detailed technical methods for studying plant PCD.
  • To enable quantitative analysis of PCD in response to developmental cues and abiotic stress.

Main Methods:

  • Root hair assay for quantitative determination of PCD and cellular injury.
  • Electrolyte leakage assay for assessing stress-induced cellular damage.
  • Lace plant model system for studying genetically regulated PCD during leaf development.

Main Results:

  • Established protocols for studying PCD in various plant contexts.
  • Demonstrated the utility of specific assays for quantitative PCD measurement.
  • Highlighted the lace plant as a valuable model for developmental PCD research.

Conclusions:

  • The presented methods offer robust approaches for investigating plant PCD.
  • These techniques facilitate a deeper understanding of plant development and stress resilience.
  • Further research using these methods can advance plant science and crop improvement.