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A Grading Score for Colon Preservation Injury in the Rat.

A Bresler1, M Ionac2, M Oltean3

  • 1Laboratory for Transplantation and Regenerative Medicine, Sahlgrenska Academy at the University of Gothenburg, Gothenburg, Sweden; University of Medicine and Pharmacy, "Victor Babes," Timisoara, Romania.

Transplantation Proceedings
|April 26, 2016
PubMed
Summary
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The colon shows greater resilience to cold ischemia than the small intestine, with minimal injury observed during prolonged cold storage. A new grading score for colon preservation injury is proposed based on these findings.

Area of Science:

  • Transplantation research
  • Gastrointestinal surgery
  • Organ preservation

Background:

  • Colon transplantation is limited by concerns of ischemic injury and septic complications.
  • Existing preservation injury scores are adapted from small intestine, overlooking organ-specific differences.
  • Systematic studies on colon preservation are lacking.

Purpose of the Study:

  • To investigate sequential tissue changes in the rat colon during prolonged cold storage (CS).
  • To develop a specific grading score for colon preservation injury.

Main Methods:

  • Rat colons were preserved in histidine-tryptophan-ketoglutarate (HTK) solution at 4°C for up to 24 hours.
  • Histological analysis included hematoxylin-eosin, alcian blue staining, and zonula occludens (ZO)-1 expression.

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  • Samples were assessed at 6, 12, 18, and 24 hours of CS.

    • Main Results:

    • Submucosal edema and Goblet cell (GC) depletion were the primary changes during CS.
    • Significant submucosal edema developed after 18 hours of CS.
    • GC depletion began between 12-18 hours, with significant depletion after 24 hours; ZO-1 expression was maintained for 18 hours.

    Conclusions:

    • The colon demonstrates higher resilience to cold ischemia compared to the small bowel.
    • Histological features of the colon are maintained longer during cold storage.
    • A novel grading score for colon preservation injury, considering submucosal edema and GC depletion, is proposed.