Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Repressible Operon: trp Operon01:21

Repressible Operon: trp Operon

2.3K
The trp operon in Escherichia coli exemplifies a repressible operon. It regulates the synthesis of tryptophan through repressor-mediated transcriptional control and attenuation. This dual regulatory mechanism ensures tryptophan biosynthesis occurs only when needed, conserving cellular resources.Structure of the trp OperonThe trp operon consists of five structural genes (trpE, trpD, trpC, trpB, and trpA) that encode enzymes for tryptophan biosynthesis. These genes are transcribed as a single...
2.3K
Reporter Genes02:11

Reporter Genes

13.7K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
13.7K
Constitutive and Regulated Gene Expression01:27

Constitutive and Regulated Gene Expression

1.6K
Gene expression in prokaryotes is governed by constitutive and regulated systems, allowing cells to balance the production of essential proteins with adaptive responses to environmental changes.Constitutive Gene ExpressionConstitutive, or housekeeping, genes are continuously expressed as they encode proteins vital for fundamental cellular processes. These include enzymes for glycolysis, ribosomal components for protein synthesis, and proteins involved in DNA replication. Their constant...
1.6K
Combinatorial Gene Control02:33

Combinatorial Gene Control

9.8K
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
9.8K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

7.3K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
7.3K
Global Regulatory Systems01:28

Global Regulatory Systems

859
Global regulatory systems in bacteria enable rapid and coordinated responses to environmental changes by integrating sensory inputs with gene expression, ensuring efficient adaptation to fluctuating conditions. Key global regulatory mechanisms include regulons, two-component systems, sigma factors, and secondary messengers.Regulons and Global RegulatorsA regulon is a collection of genes and operons controlled by a common global regulator. These regulators enable bacteria to prioritize resource...
859

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Frequency-Guided Cross-Modal Interaction for Multimodal Yeast Classification Based on Light-Scattering and Microscopy Images.

Journal of imaging·2026
Same author

Gene loss and compensatory evolution as a source of metabolic preadaptations.

Molecular biology and evolution·2026
Same author

Prevalent gut phages encode modular adhesins mediating epithelial binding and endoplasmic reticulum trafficking.

Nature communications·2026
Same author

Peering Inside the Black Box: Explainable AI to Interpret Advanced Computer Vision Fungal Pathogen Prediction.

Scientific reports·2026
Same author

Machine Learning-Assisted Classification of Pathogenic Yeasts Using Laser Light Scattering and Conventional Microscopy.

Journal of imaging·2026
Same author

An ATP-Mediated Antibiotic β-Peptide Nanofiber That Kills Multidrug-Resistant Bacteria via a Multistage Mechanism.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Related Experiment Video

Updated: Mar 22, 2026

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells
09:20

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells

Published on: July 6, 2021

2.9K

Efflux Pump Control Alters Synthetic Gene Circuit Function.

Junchen Diao1, Daniel A Charlebois2, Dmitry Nevozhay1,3

  • 1Department of Systems Biology, The University of Texas MD Anderson Cancer Center , Unit 950, 7435 Fannin Street, Houston, Texas 77054, United States.

ACS Synthetic Biology
|April 26, 2016
PubMed
Summary
This summary is machine-generated.

Synthetic biology uses gene circuits to control cellular functions. Researchers found that efflux pumps, designed to secrete products, can unexpectedly alter gene circuit behavior by removing inducers, requiring mathematical modeling for accurate predictions.

Keywords:
efflux pumpfeedbackstochastic simulationsynthetic gene circuit

More Related Videos

Immunometabolic Circuits in Infection for Advancing Host Directed Therapies
11:12

Immunometabolic Circuits in Infection for Advancing Host Directed Therapies

Published on: September 13, 2024

1.0K
A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression
11:23

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression

Published on: October 6, 2019

10.9K

Related Experiment Videos

Last Updated: Mar 22, 2026

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells
09:20

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells

Published on: July 6, 2021

2.9K
Immunometabolic Circuits in Infection for Advancing Host Directed Therapies
11:12

Immunometabolic Circuits in Infection for Advancing Host Directed Therapies

Published on: September 13, 2024

1.0K
A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression
11:23

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression

Published on: October 6, 2019

10.9K

Area of Science:

  • Synthetic biology
  • Systems biology
  • Biotechnology

Background:

  • Synthetic gene circuits are designed for controlled cellular functions.
  • ATP-binding cassette (ABC) efflux pumps can secrete molecules but may also remove gene circuit inducers.

Purpose of the Study:

  • To investigate how ABC efflux pumps affect synthetic gene circuit dynamics.
  • To understand the feedback mechanisms introduced by efflux pumps in Saccharomyces cerevisiae.

Main Methods:

  • Utilized two synthetic gene circuits responsive to tetracycline-family inducers.
  • Regulated the expression of a yeast ABC pump (Pdr5p) known to efflux inducers.
  • Employed quantitative modeling to analyze dose-response alterations.
  • Performed experimental validation of model predictions.

Main Results:

  • Pdr5p significantly altered the dose-responses of the synthetic gene circuits.
  • Both the efflux function and reduced regulator gene expression contributed to the observed changes.
  • Mathematical modeling accurately predicted the altered gene circuit behavior.

Conclusions:

  • Efflux pumps can introduce complex, implicit feedback loops that modify synthetic gene circuit performance.
  • Quantitative modeling is crucial for predicting and understanding these dynamics in synthetic biology.
  • This study provides insights into designing more robust synthetic gene circuits.