Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A highly efficient method to differentiate CGRP-expressing peptidergic nociceptors from human induced pluripotent stem cells.

Stem cell reports·2026

Same author

Identification of genetic risk loci associated with aquaporin 4-positive neuromyelitis optica spectrum disorder: a genome-wide association study.

The Lancet. Neurology·2026

Same author

MOGAD in South Wales: Diagnostic Evolution and Disease Epidemiology.

European journal of neurology·2026

Same author

Optimal strategies for treatment discontinuation in MOG antibody-associated disease.

Brain : a journal of neurology·2026

Same author

Clinical Criteria to Guide Antineuronal Antibody Testing for People With Early and Persistent Psychosis Attending Mental Health Services.

Biological psychiatry global open science·2025

Same author

Quantitative analysis of aquaporin-4 (AQP4) and myelin oligodendrocyte glycoprotein (MOG) antibodies titres: correlation with relapses.

Brain communications·2025

Same journal

Preface.

Handbook of clinical neurology·2026

Same journal

Foreword.

Handbook of clinical neurology·2026

Same journal

Fundus autofluorescence imaging.

Handbook of clinical neurology·2026

Same journal

The electroretinogram as a means to study the physiology of the retina.

Handbook of clinical neurology·2026

Same journal

Adaptive optics scanning light ophthalmoscopy.

Handbook of clinical neurology·2026

Same journal

Modeling the human retina in a dish: Advances and future directions.

Handbook of clinical neurology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jan 19, 2026

Hippocampal Neuronal Cultures to Detect and Study New Pathogenic Antibodies Involved in Autoimmune Encephalitis

Hippocampal Neuronal Cultures to Detect and Study New Pathogenic Antibodies Involved in Autoimmune Encephalitis

Published on: June 2, 2022

Detection methods for neural autoantibodies.

Patrick Waters¹, Philippa Pettingill², Bethan Lang¹

¹Neuroimmunology Group, Nuffield Department of Clinical Neurosciences, John Radcliffe Hospital, Oxford, UK.

Handbook of Clinical Neurology

|April 27, 2016

Summary

This summary is machine-generated.

This study differentiates antibodies targeting central nervous system structures. It explains detection methods for intracellular biomarker antibodies and extracellular antibodies recognizing native proteins, crucial for diagnosing neurological conditions and cancers.

Keywords:

CNS ELISA IgG antibody assay cell-based assay flow cytometry immunoglobulin immunohistochemistry immunoprecipitation

More Related Videos

A Simple Cell-based Immunofluorescence Assay to Detect Autoantibody Against the N-Methyl-D-Aspartate NMDA Receptor in Blood

A Simple Cell-based Immunofluorescence Assay to Detect Autoantibody Against the N-Methyl-D-Aspartate NMDA Receptor in Blood

Published on: January 9, 2018

High-throughput Flow Cytometry Cell-based Assay to Detect Antibodies to N-Methyl-D-aspartate Receptor or Dopamine-2 Receptor in Human Serum

High-throughput Flow Cytometry Cell-based Assay to Detect Antibodies to N-Methyl-D-aspartate Receptor or Dopamine-2 Receptor in Human Serum

Published on: November 23, 2013

Related Experiment Videos

Last Updated: Jan 19, 2026

Hippocampal Neuronal Cultures to Detect and Study New Pathogenic Antibodies Involved in Autoimmune Encephalitis

Hippocampal Neuronal Cultures to Detect and Study New Pathogenic Antibodies Involved in Autoimmune Encephalitis

Published on: June 2, 2022

A Simple Cell-based Immunofluorescence Assay to Detect Autoantibody Against the N-Methyl-D-Aspartate NMDA Receptor in Blood

A Simple Cell-based Immunofluorescence Assay to Detect Autoantibody Against the N-Methyl-D-Aspartate NMDA Receptor in Blood

Published on: January 9, 2018

High-throughput Flow Cytometry Cell-based Assay to Detect Antibodies to N-Methyl-D-aspartate Receptor or Dopamine-2 Receptor in Human Serum

High-throughput Flow Cytometry Cell-based Assay to Detect Antibodies to N-Methyl-D-aspartate Receptor or Dopamine-2 Receptor in Human Serum

Published on: November 23, 2013

Area of Science:

Neuroimmunology
Oncoimmunology

Background:

Antibodies are key to adaptive immunity but can target central nervous system (CNS) structures.
Antibodies are classified by target location: intracellular (cytosolic/nuclear) or extracellular (native cell surface proteins).

Purpose of the Study:

To differentiate between antibodies targeting intracellular vs. extracellular CNS antigens.
To describe methods for detecting both antibody types and discuss practical aspects of testing.

Main Methods:

Detection of intracellular antibodies uses assays like Western blots and line blots with denatured targets.
Detection of extracellular antibodies requires newer assays preserving native protein structures.

Main Results:

Intracellular antibodies often serve as paraneoplastic biomarkers for cancer screening.
Extracellular antibodies target native protein structures, indicating distinct autoimmune or neurological processes.

Conclusions:

Assay methodologies must align with antibody target characteristics (denatured vs. native).
Proper detection and interpretation of these antibodies are vital for clinical diagnosis and patient management.