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Updated: Mar 22, 2026

Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants
Published on: August 5, 2020
RNA sequencing analysis of gene expression regulated by the transcription factor SlZFP2 during early fruit
Lin Weng1, Fangfang Zhao1, Rong Li1
1National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Rd, Shanghai 200032, China.
Abstract:
The transcription factor SlZFP2 (Solanum lycopersicum Zinc Finger Protein 2) regulates ABA biosynthesis during fruit development. To reveal the regulatory network of this transcription factor, we conducted a high-throughput RNA-seq to identify differentially expressed genes in 2 dpa (days post anthesis) fruits from a representative RNAi line in Solanum pimpinellifolium LA1589 background and the wild type. The transcriptome analysis revealed that expression of 2722 genes was regulated by SlZFP2 during early fruit development and further helped to narrow down its direct targets to 193 genes. Here, we provide a detailed description of the experimental procedure associated with our transcriptome sequencing data deposited in the National Center for Biotechnology Information (accession no. GSE63838).
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