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Updated: Mar 22, 2026

Preparation and Immunostaining of Myelinating Organotypic Cerebellar Slice Cultures
Published on: March 20, 2019
Data supporting the role of Fyn in initiating myelination in the peripheral nervous system
Yuki Miyamoto1, Moe Tamano2, Tomohiro Torii1
1Department of Pharmacology, National Research Institute for Child Health and Development, Setagaya, Tokyo 157-8535, Japan.
Abstract:
Transgenic mice, which express active Fyn tyrosine kinase under the control of a glial fibrillary acidic protein promoter, have been produced. This promoter induces protein expression in the initiation stage of myelination in the peripheral nervous system (PNS) "Phosphorylation of cytohesin-1 by Fyn is required for initiation of myelination and the extent of myelination during development (Yamauchi et al., 2015 [1])". Herein we provide the data regarding myelination-related protein markers and myelin ultrastructure in transgenic mice.
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