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Optimization of cAMP fluorescence dataset from ACTOne cannabinoid receptor 1 cell line
Chaela S Presley1, Ammaar H Abidi1, Bob M Moore1
1University of Tennessee Health Science Center, College of Pharmacy, United States.
Abstract:
The ACTOne cannabinoid receptor 1 functional system is comprised of transfected HEK cells with the parental cyclic nucleotide gated channel (CNG) co-transfected with cannabinoid receptor 1 (CB1). The ACTOne CB1 cell line was evaluated for cAMP driven fluorescence by optimizing experimental conditions for sensitivity to forskolin and CP 55,940, reading time point, reliability of cell passage number, and pertussis inactivation of Gi/o.
