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Developmental exposure to acetaminophen does not induce hyperactivity in zebrafish larvae.

Isabel Reuter^1,2, Sabine Knaup³, Marcel Romanos⁴

¹Department of Physiological Chemistry, Biocenter, Am Hubland, University of Würzburg, 97074, Würzburg, Germany.

Journal of Neural Transmission (Vienna, Austria : 1996)

|April 27, 2016

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Hyperactivity and Differential Gene Expression in <i>lbx1a<sup>(</sup><sup>-/</sup><sup>-)</sup></i> Zebrafish Larvae.

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Prenatal acetaminophen exposure in zebrafish did not increase hyperactivity in larvae. This study suggests no direct link between embryonic acetaminophen exposure and attention-deficit/hyperactivity disorder (ADHD)-like symptoms in this model.

Area of Science:

Neurodevelopmental disorders
Pharmacology
Zebrafish models

Background:

Acetaminophen is a common pain reliever during pregnancy.
Epidemiological studies link prenatal acetaminophen use to ADHD-like symptoms in children.
Experimental evidence for this link is lacking.

Purpose of the Study:

To investigate if sub-liver-toxic concentrations of acetaminophen during embryonic development cause hyperactivity in zebrafish.
To examine if acetaminophen exposure exacerbates hyperactivity in a zebrafish model of ADHD (lphn3.1 knockdown).

Main Methods:

Determined sub-liver-toxic acetaminophen concentrations in zebrafish larvae.
Exposed wild-type and lphn3.1 knockdown zebrafish larvae to acetaminophen.
Assessed locomotor activity levels as a measure of hyperactivity.

Keywords:

Acetaminophen Behavior Impulsivity Locomotion latrophilin3.1

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Main Results:

lphn3.1 knockdown alone caused hyperactivity, validating the ADHD model.
Acetaminophen exposure, at sub-liver-toxic levels, did not increase locomotor activity in wild-type or lphn3.1 knockdown larvae.
No additive or synergistic effects of acetaminophen were observed in the lphn3.1 knockdown model.

Conclusions:

Embryonic exposure to acetaminophen does not appear to cause hyperactivity in zebrafish larvae.
The study found no direct link between prenatal acetaminophen exposure and hyperactivity in this zebrafish model.
Further research is needed to confirm these findings in humans.