Related Experiment Video
Updated: Mar 22, 2026
Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
New Insights for Diagnosis of Pineapple Fusariosis by MALDI-TOF MS Technique
Cledir Santos1, José Aires Ventura2, Nelson Lima3
1Department of Chemical Sciences and Natural Resources, CIBAMA, BIOREN-UFRO, Faculty of Engineering and Science, Universidad de La Frontera, Av. Francisco Salazar 01145, 4811-230, Temuco, Chile. cledir.santos@ufrontera.cl.
Abstract:
Fusarium is one of the most economically important fungal genus, since it includes many pathogenic species which cause a wide range of plant diseases. Morphological or molecular biology identification of Fusarium species is a limiting step in the fast diagnosis and treatment of plant disease caused by these fungi. Mass spectrometry by matrix-assisted laser/desorption ionisation-time-of-flight (MALDI-TOF)-based fingerprinting approach was applied to the fungal growth monitoring and direct detection of strain Fusarium guttiforme E-480 inoculated in both pineapple cultivars Pérola and Imperial side shoots, that are susceptible and resistant, respectively, to this fungal strain. MALDI-TOF MS technique was capable to detect fungal molecular mass peaks in the susceptible pineapple stem side shoot tissue. It is assumed that these molecular masses are mainly constituted by ribosomal proteins. MALDI-TOF-based fingerprinting approach has herein been demonstrated to be sensitive and accurate for the direct detection of F. guttiforme E-480 molecular masses on both susceptible and resistant pineapple side stem free of any pre-treatment. According to the results obtained, the changing on molecular mass peaks of infected susceptible pineapple tissue together with the possibility of fungal molecular masses analysis into this pineapple tissue can be a good indication for an early diagnosis by MALDI-TOF MS of pineapple fusariosis.
Insights
Matrix-assisted laser/desorption ionisation-time-of-flight (MALDI-TOF) mass spectrometry rapidly detects Fusarium guttiforme in pineapple. This fungal detection method aids in early diagnosis of pineapple fusariosis.
Area of Science:
- Agricultural Science
- Mycology
- Plant Pathology
Background:
- Fusarium species are significant plant pathogens causing substantial economic losses.
- Accurate and rapid identification of Fusarium is crucial for timely plant disease management.
- Current identification methods can be time-consuming, limiting effective treatment.
Purpose of the Study:
- To evaluate the efficacy of MALDI-TOF MS for direct detection of Fusarium guttiforme E-480 in pineapple.
- To assess the potential of MALDI-TOF MS for early diagnosis of pineapple fusariosis.
- To monitor fungal growth in susceptible and resistant pineapple cultivars.
Main Methods:
- Matrix-assisted laser/desorption ionisation-time-of-flight (MALDI-TOF) mass spectrometry was employed.
- The technique was applied to detect Fusarium guttiforme E-480 in inoculated pineapple (cultivars Pérola and Imperial) side shoots.
- Direct detection in plant tissue without pre-treatment was performed.
Main Results:
- MALDI-TOF MS successfully detected characteristic fungal molecular mass peaks in susceptible pineapple stem tissue.
- The technique demonstrated sensitivity and accuracy in detecting F. guttiforme E-480 molecular masses.
- Changes in molecular mass peaks within infected tissue suggest potential for early diagnosis.
Conclusions:
- MALDI-TOF MS is a viable tool for the direct and rapid detection of Fusarium guttiforme in pineapple.
- The technique shows promise for early diagnosis of pineapple fusariosis, enabling prompt intervention.
- MALDI-TOF MS can differentiate between susceptible and resistant pineapple responses to the pathogen.
More Related Videos
07:18Natural Product Discovery with LC-MS/MS Diagnostic Fragmentation Filtering: Application for Microcystin Analysis
Published on: May 31, 2019
09:38Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
Published on: November 26, 2013
Related Concept Videos
01:19MALDI-TOF Mass Spectrometry
01:33Peptide Identification Using Tandem Mass Spectrometry
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...