Phenylobacterium panacis sp. nov., isolated from the rhizosphere of rusty mountain ginseng

A novel, Gram-stain-negative, rod-shaped bacterial strain, designated as DCY109^T, was isolated from the rhizosphere of rusty mountain ginseng root located on Hwacheon mountain of Gangwon province, South Korea. 16S rRNA gene sequence analysis revealed that strain DCY109^T belonged to the genus Phenylobacterium and was related closely to Phenylobacterium muchangponense KACC 15042^T (98.2 % similarity), Phenylobacterium immobile DSM 1986^T (96.9 %) and Phenylobacterium koreense KCTC 12206^T (96.7 %). The predominant isoprenoid quinone was ubiquinone (Q-10) and the DNA G+C content was 66.9 mol%. The major polar lipids were phosphatidylglycerol, an unidentified glycolipid and an unidentified lipid. The major fatty acids (>10 %) were C16 : 0, summed feature 3 (which comprised C16 : 1ω7c and/or C16 : 1ω6c) and summed feature 8 (which comprised C18 : 1ω7c and/or C18 : 1ω6c). Mean DNA-DNA relatedness between strain DCY109^T and its closest relative, P. muchangponense KACC 15042^T, was 15.1±3.9 %. Based on the physiological, biochemical, chemotaxonomic and genetic analyses, strain DCY109^T is considered to represent a novel species of the genus Phenylobacterium, for which the name Phenylobacterium panacis sp. nov. is proposed. The type strain is DCY109^T (=KCTC 42749^T=JCM 31045^T).