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The Microfluidic Probe: Operation and Use for Localized Surface Processing
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Diffusing colloidal probes of cell surfaces.

Gregg A Duncan1, D Howard Fairbrother2, Michael A Bevan1

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This study quantifies interactions between colloidal particles and cancer cells using microscopy. Polyethylene glycol (PEG) coatings stabilize particles on cell surfaces via repulsive forces, revealing cell topography.

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Area of Science:

  • Biophysics
  • Materials Science
  • Cell Biology

Background:

  • Understanding cell surface biophysics is crucial for drug delivery and diagnostics.
  • Colloidal particle interactions with cell surfaces are complex and require precise measurement.
  • Polyethylene glycol (PEG) coatings are widely used to modify particle properties.

Purpose of the Study:

  • To quantify dynamic and equilibrium interactions between colloidal particles and live cancer cell surfaces.
  • To investigate the role of PEG coatings in colloidal particle-cell surface interactions.
  • To characterize biophysical cell surface properties using colloidal probes.

Main Methods:

  • Dark field video microscopy to track colloidal particle trajectories.
  • Analysis of two-dimensional particle movement relative to cell perimeters.
  • Measurement of position-dependent diffusivities and interaction potentials.
  • Modeling of hydrodynamic interactions between particles and cell surfaces.

Main Results:

  • PEG-coated silica colloids exhibit stabilized interactions on cell surfaces due to steric repulsion.
  • Analysis of potential energies revealed spatial dependence on cell topography.
  • Excellent agreement between theoretical models and measured colloidal transport.
  • Quantitative analysis of association lifetimes confirmed PEG's stabilizing effect.

Conclusions:

  • PEG coatings provide repulsive steric interactions, stabilizing colloids on cell surfaces.
  • Colloidal probe measurements accurately characterize cell surface topography and biophysical properties.
  • This work provides a foundation for studying specific particle-cell interactions.