Related Experiment Video
Updated: Mar 22, 2026
Analyzing Oxidative Stress in Murine Intestinal Organoids using Reactive Oxygen Species-Sensitive Fluorogenic Probe
Published on: September 17, 2021
Oxidative stress controlling agents are effective for small intestinal injuries induced by non-steroidal
Yoshiyasu Kono1, Seiji Kawano1, Akinobu Takaki1
1Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
Background And Aim:
Video-capsule endoscopy (VCE) has shown that intestinal ulcers are common in non-steroidal anti-inflammatory drugs (NSAIDs) users, although the mechanisms and management have not been clearly defined. To explore the contribution of oxidative stress and potential of anti-oxidants for NSAIDs-induced intestinal ulcers, we assessed human serum oxidative stress balance and the effect of anti-oxidants using a mouse model.
Methods:
A total of 30 NSAIDs users (17 aspirin and 13 non-aspirin users) received VCE. Serum reactive oxygen metabolite (d-ROM) and antioxidative OXY-adsorbent test (OXY) were measured. The indomethacin (IND)-induced mouse intestinal ulcer model was used to assess the effect of anti-oxidants. Eight-week-old mice were divided into four groups; control diet and diet including IND (N group), IND and L-carnitine (NC group), and IND and vitamin E (NE group).
Results:
Serum OXY levels among non-aspirin users were lower in the mucosal injuries positive group than the negative group (P < 0.05). In the mouse models, the degree of mucosal injuries was lower in NC and NE than N (P < 0.01). Serum d-ROM levels were lower in NC and NE than N (P < 0.01), and OXY levels were higher in NC than N and NE (P < 0.01). The degeneration of intestinal mitochondria was mild in NC and NE. The serum KC/CXCL-1 level and hepatic expression of the anti-oxidant molecule Gpx4 were lower in NC than N.
Conclusions:
Non-aspirin NSAID-induced intestinal ulcers are related to decreased anti-oxidative stress function. Anti-oxidants, especially L-carnitine, are good candidates for intestinal ulcers.
Insights
Non-aspirin NSAID use is linked to reduced antioxidant function, causing intestinal ulcers. Antioxidants like L-carnitine show promise in preventing and treating these ulcers.
Area of Science:
- Gastroenterology
- Pharmacology
- Oxidative Stress Research
Background:
- Non-steroidal anti-inflammatory drugs (NSAIDs) are frequently associated with intestinal ulcers, yet the underlying mechanisms and effective management strategies remain incompletely understood.
- Video-capsule endoscopy (VCE) has revealed a high prevalence of intestinal ulcers in NSAID users, highlighting the need for further investigation into causative factors and therapeutic interventions.
Purpose of the Study:
- To investigate the role of oxidative stress in NSAID-induced intestinal ulcers.
- To evaluate the potential therapeutic effects of antioxidants in mitigating NSAID-induced intestinal damage.
Main Methods:
- Human subjects (30 NSAID users) underwent VCE, with serum oxidative stress markers (d-ROM and OXY) measured.
- An indomethacin (IND)-induced mouse intestinal ulcer model was employed, with mice assigned to control, IND, IND + L-carnitine (NC), and IND + vitamin E (NE) groups.
Main Results:
- NSAID users with intestinal mucosal injuries exhibited lower serum OXY levels.
- In the mouse model, L-carnitine (NC) and vitamin E (NE) groups showed significantly reduced intestinal injury compared to the IND group (N).
- Antioxidant treatment (NC and NE) improved serum oxidative balance (lower d-ROM, higher OXY) and reduced mitochondrial degeneration.
Conclusions:
- NSAID-induced intestinal ulcers are associated with impaired antioxidant capacity.
- Antioxidants, particularly L-carnitine, demonstrate significant potential as therapeutic agents for NSAID-induced intestinal ulcers.
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