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Updated: Mar 22, 2026

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
Published on: August 16, 2016
Driven Translocation of Polynucleotides Through an Aerolysin Nanopore
Chan Cao1, Jie Yu1, Ya-Qian Wang1
1Key Laboratory for Advanced Materials and Department of Chemistry, East China University of Science and Technology , Shanghai 200237, P. R. China.
Abstract:
Aerolysin has been used as a biological nanopore for studying peptides, proteins, and oligosaccharides in the past two decades. Here, we report that wild-type aerolysin could be utilized for polynucleotide analysis. Driven a short polynucleotide of four nucleotides length through aerolysin occludes nearly 50% amplitude of the open pore current. Furthermore, the result of total internal reflection fluorescence measurement provides direct evidence for the driven translocation of single polynucleotide through aerolysin.
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