Related Experiment Video

Updated: Mar 22, 2026

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
07:02

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

7.9K

PATZ1 is a target of miR-29b that is induced by Ha-Ras oncogene in rat thyroid cells

Michela Vitiello1, Teresa Valentino1, Marta De Menna2

  • 1Institute of Experimental Endocrinology and Oncology (IEOS), National Research Council (CNR), 80131 Naples, Italy.

Scientific Reports
|April 30, 2016
PubMed

Insights

The tumor suppressor PATZ1 is downregulated in thyroid cancer. Ras oncogene upregulates miR-29b, which targets PATZ1, promoting cancer cell proliferation and migration.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Regulation

Background:

  • The transcriptional factor PATZ1 functions as a tumor suppressor in human thyroid cancer.
  • PATZ1 targets p53-dependent genes involved in Epithelial-Mesenchymal Transition (EMT) and cell migration.
  • Understanding the regulation of PATZ1 expression is crucial for thyroid cancer research.

Purpose of the Study:

  • To investigate the upstream signaling mechanisms that regulate PATZ1 expression in thyroid cancer cells.
  • To identify microRNAs (miRNAs) targeting PATZ1.
  • To elucidate the role of miR-29b in Ras-driven thyroid cancer.

Main Methods:

  • Bioinformatic analysis to identify potential miRNA targets of PATZ1.
  • Functional assays to confirm PATZ1 as a target of miR-29b.
  • In vitro studies using rat thyroid cells expressing the Ha-Ras oncogene.

Main Results:

  • miR-29b was identified as a key regulator targeting PATZ1.
  • An inverse correlation between miR-29b and PATZ1 protein levels was observed upon Ha-Ras oncogene induction.
  • Restoration of PATZ1 expression reduced proliferation and migration in Ras-transformed thyroid cells.

Conclusions:

  • Ras oncogene upregulates miR-29b expression, leading to PATZ1 downregulation.
  • This miR-29b-mediated downregulation of PATZ1 plays a significant role in Ras-driven thyroid transformation.
  • PATZ1 acts as a crucial tumor suppressor in the context of Ras-induced thyroid cancer.

More Related Videos

Spontaneous Murine Model of Anaplastic Thyroid Cancer05:39

Spontaneous Murine Model of Anaplastic Thyroid Cancer

Published on: February 3, 2023

2.4K
Generation of Hypoparathyroid Rats via Carbon-Nanoparticle-Assisted Parathyroidectomy03:57

Generation of Hypoparathyroid Rats via Carbon-Nanoparticle-Assisted Parathyroidectomy

Published on: July 14, 2023

2.4K

Related Concept Videos

PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
6.2K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
5.0K
The Ras Gene02:38

The Ras Gene

The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a...
7.5K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.4K
Catenins01:23

Catenins

Catenins are characterized by multiple binding domains and dynamic structures that allow them to function as linker proteins in cell junction complexes. All catenins, except α-catenin, contain a characteristic protein sequence called the armadillo repeat and are therefore also called armadillo proteins.
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the...
3.2K