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CRISPR/Cas9 Genome Editing

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

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Related Experiment Video

Updated: Mar 22, 2026

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9

Published on: November 3, 2020

In vitro CRISPR-Cas9-mediated efficient Ad5 vector modification.

Lichun Tang¹, Mengmeng Gong², Pumin Zhang¹

¹State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, China; National Center for Protein Sciences Beijing, Life Sciences Park, Beijing 102206, China.

Biochemical and Biophysical Research Communications

|April 30, 2016

Summary

This summary is machine-generated.

A new CRISPR-Cas9 system edits large DNA vectors in vitro. This genome editing tool efficiently generates targeted mutations, deletions, and replacements in Adenovirus 5 vectors for diverse research applications.

Keywords:

Ad5/F35 Adenovirus CRISPR-Cas9 Fiber modification

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Area of Science:

Molecular Biology
Genetics
Biotechnology

Background:

The CRISPR-Cas9 system is a powerful tool for genome editing across various organisms.
Existing methods for large DNA vector editing can be complex and time-consuming.

Purpose of the Study:

To develop and validate an in vitro CRISPR-Cas9 based system for editing large DNA vectors.
To demonstrate the system's capability in generating specific genetic modifications within Adenovirus 5 vectors.

Main Methods:

Utilized the CRISPR-Cas9 system for in vitro editing of Adenovirus 5 based vectors.
Applied the system to introduce targeted mutations, in-frame gene deletions, and gene replacements.

Main Results:

The developed CRISPR-Cas9 system demonstrated high efficiency in editing large DNA vectors.
The system accurately generated targeted mutations, in-frame gene deletions, and gene replacements.
Successful application was shown using Adenovirus 5 vectors as a model system.

Conclusions:

The in vitro CRISPR-Cas9 vector editing system offers a highly efficient and accurate method for genetic modification.
This system holds potential for broad application in various experimental and research settings.
Facilitates precise manipulation of large DNA constructs for biological studies.