Algicidal and denitrification characterization of Acinetobacter sp. J25 against Microcystis aeruginosa and microbial community in eutrophic landscape water
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Updated: Mar 22, 2026

Author Spotlight: A Comprehensive Protocol for Acinetobacter Biofilm Quantification, Assessment, and Visualization
Published on: August 4, 2023
Jun Feng Su1, Min Ma2, Li Wei3
1School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, People's Republic of China; State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, People's Republic of China.
Abstract:
Acinetobacter sp. J25 exhibited good denitrification and high algicidal activity against toxic Microcystis aeruginosa. Response surface methodology (RSM) experiments showed that the maximum algicidal ratio occurred under the following conditions: temperature, 30.46°C; M. aeruginosa density, 960,000cellsmL(-1); and inoculum, 23.75% (v/v). Of these, inoculum produced the maximum effect. In the eutrophic landscape water experiment, 10% bacterial culture was infected with M. aeruginosa cells in the landscape water. After 24days, the removal ratios of nitrate and chlorophyll-a were high, 100% and 87.86%, respectively. The denitrification rate was approximately 0.118mgNO3(-)-N·L(-1)·h(-1). Moreover, the high-throughput sequencing result showed that Acinetobacter sp. J25 was obviously beneficial for chlorophyll-a and nitrate removal performance in the eutrophic landscape water treatment. Therefore, strain J25 is promising for the simultaneous removal of chlorophyll-a and nitrate in the eutrophic landscape water treatment.
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