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Author Spotlight: A Comprehensive Protocol for Acinetobacter Biofilm Quantification, Assessment, and Visualization
Published on: August 4, 2023
Algicidal and denitrification characterization of Acinetobacter sp. J25 against Microcystis aeruginosa and microbial
Jun Feng Su1, Min Ma2, Li Wei3
1School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, People's Republic of China; State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, People's Republic of China.
Abstract:
Acinetobacter sp. J25 exhibited good denitrification and high algicidal activity against toxic Microcystis aeruginosa. Response surface methodology (RSM) experiments showed that the maximum algicidal ratio occurred under the following conditions: temperature, 30.46°C; M. aeruginosa density, 960,000cellsmL(-1); and inoculum, 23.75% (v/v). Of these, inoculum produced the maximum effect. In the eutrophic landscape water experiment, 10% bacterial culture was infected with M. aeruginosa cells in the landscape water. After 24days, the removal ratios of nitrate and chlorophyll-a were high, 100% and 87.86%, respectively. The denitrification rate was approximately 0.118mgNO3(-)-N·L(-1)·h(-1). Moreover, the high-throughput sequencing result showed that Acinetobacter sp. J25 was obviously beneficial for chlorophyll-a and nitrate removal performance in the eutrophic landscape water treatment. Therefore, strain J25 is promising for the simultaneous removal of chlorophyll-a and nitrate in the eutrophic landscape water treatment.
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