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Ratiometric mechanosensitive fluorescent dyes: Design and applications.

Mark A Haidekker1, Emmanuel A Theodorakis2

  • 1College of Engineering, University of Georgia, 597 D. W. Brooks Drive, Athens, GA 30602, USA.

Journal of Materials Chemistry. C
|April 30, 2016
PubMed
Summary
This summary is machine-generated.

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Fluorescent molecular rotors are advanced mechanosensors that measure microviscosity. Dual-emission designs overcome limitations of traditional fluorescent probes, enabling more accurate sensing applications.

Area of Science:

  • Analytical Chemistry
  • Materials Science
  • Biophysics

Background:

  • Fluorescent molecules are valuable analytical tools and biosensors due to their rapid response and low-cost instrumentation.
  • Recent advances in chemical synthesis have led to the development of sophisticated fluorescent probes, including molecular rotors.

Purpose of the Study:

  • To focus on fluorescent molecular rotors, a class of mechanosensors whose emission quantum yield correlates with microviscosity.
  • To discuss the development and application of dual-emission molecular rotors designed to mitigate confounding factors in fluorescence measurements.

Main Methods:

  • Exploration of the theory and chemical structure of fluorescent molecular rotors.
  • Analysis of dual-emission strategies, including intrinsic dual emission and reference unit incorporation.

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  • Review of targeted design principles for novel molecular rotor development.

    • Main Results:

    • Fluorescent molecular rotors exhibit a relationship between emission quantum yield and microviscosity.
    • Dual-emission dyes, including molecular rotors with intrinsic dual emission or reference bands, can overcome limitations of steady-state fluorescence intensity measurements.
    • Development of specific classes of dual-emission molecular rotors is reported.

    Conclusions:

    • Dual-emission molecular rotors offer enhanced capabilities for microviscosity sensing compared to traditional fluorescent probes.
    • These advanced probes provide a more reliable method for measuring microviscosity by decoupling emission intensity from quantum yield.
    • The reported designs and applications highlight the potential of molecular rotors in various sensing fields.