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All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...

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The use of SC1 Pluripotin to Support mESC Self-renewal in the Absence of LIF

The use of SC1 Pluripotin to Support mESC Self-renewal in the Absence of LIF

Published on: November 18, 2009

LIF signal in mouse embryonic stem cells.

Satoshi Ohtsuka¹, Yoko Nakai-Futatsugi¹, Hitoshi Niwa²

¹Laboratory for Pluripotent Stem Cell Studies; Center for Developmental Biology (CDB) RIKEN ; Kobe, Japan.

|April 30, 2016

Summary

Leukemia inhibitory factor (LIF) is crucial for mouse embryonic stem cell pluripotency in conventional culture. However, its necessity diminishes in 2i-culture, suggesting context-dependent roles in maintaining naïve pluripotency.

Keywords:

Embryonic stem cell (ESC), Lekemia inhibitory factor (LIF) signal, Stat3, MAP kinase, PI3K-Akt, Genetic background, naive state of pluripoetncy, Epigenetics

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Area of Science:

Stem cell biology
Cellular signaling

Background:

Mouse embryonic stem cells (mESCs) were established in the 1980s, advancing understanding of in vitro pluripotent stem cell self-renewal.
Leukemia inhibitory factor (LIF) is essential for pluripotency in conventional serum-containing media.
In serum-free 2i-culture (inhibiting Map-kinase and Gsk3), LIF is not required.

Purpose of the Study:

To investigate the context-dependent role of LIF in maintaining naïve pluripotency.
To explore how the LIF-signal pathway contributes to the naïve pluripotency state.

Main Methods:

Review of existing literature on mESC culture conditions and pluripotency maintenance.
Analysis of signaling pathways involved in LIF-dependent and independent pluripotency.

Main Results:

LIF is essential in conventional culture but dispensable in 2i-culture for pluripotency.
Emerging evidence indicates LIF may play a role beyond 2i inhibition for naïve pluripotency.
LIF signaling converges to maintain the naïve state in a context-dependent manner.

Conclusions:

LIF's function in maintaining naïve pluripotency is highly dependent on the culture context.
Understanding LIF's signaling pathways is key to elucidating pluripotency maintenance mechanisms.