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Updated: Mar 22, 2026
Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides
Published on: July 14, 2015
Preparation of Ti(4+)-immobilized modified silica capillary trapping column for on-line selective enrichment of
Huili Yang1, Chunhui Deng1, Xiangmin Zhang1
1Department of Chemistry and Institutes of Biomedical Science, Fudan University, Shanghai 200433, People's Republic of China.
Abstract:
In this work, we have developed Ti(4+)-immobilized capillary trapping column (250μm i.d.) for highly specific on-line enrichment of phosphopeptides in the bio-samples. It minimizes the sample loss and improves the effectiveness of phosphopeptide detection that the limit of detection is as low as 1fmol/μL. It is successfully applied to the detection of phosphopeptides from complex biological samples, such as skim milk, human serum and mouse brain. The results indicate that the Ti(4+)-immobilized capillary trapping column is time-effective and has the great potential of application in low-abundance phosphopeptides on-line analysis. The prepared Ti(4+)-immobilized capillary trapping column will be further used in LC/MS platform for phosphoproteome analysis.
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