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SS-B (La) nuclear antigen: fast and non-degradative procedure to prepare SS-B extracts free from other nuclear
Biological Chemistry Hoppe-Seyler
|January 1, 1989
Abstract:
A procedure is described allowing the easy and fast obtention of a cellular extract from calf thymus, enriched in the undegraded 52 kDa SS-B protein. As seen by western blot, the extract does not contain Sm and RNP antigens, allowing the use of such fraction for the detection of anti-SS-B antibodies without the interference of the anti-Sm and anti-RNP specificities. The enrichment avoids denaturing agents, making the fraction suitable for use in functional and structural studies.