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A versatile fluorescent biosensor based on target-responsive graphene oxide hydrogel for antibiotic detection.

Bing Tan1, Huimin Zhao1, Lei Du1

  • 1Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education, China), School of Environmental Science and Technology, Dalian University of Technology, Linggong Road 2, Dalian 116024, PR China.

Biosensors & Bioelectronics
|May 2, 2016
PubMed
Summary

A novel graphene oxide hydrogel was developed for sensitive detection of oxytetracycline (OTC). This platform offers a robust and versatile method for analyzing various molecules in analytical chemistry.

Keywords:
AntibioticAptamerFluorescent detectionGO hydrogelOxytetracyclineSulfadimethoxine

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Area of Science:

  • Materials Science
  • Analytical Chemistry
  • Biotechnology

Background:

  • Graphene oxide (GO) hydrogels offer unique properties for material development.
  • Developing sensitive and selective detection platforms is crucial for analytical applications.
  • Aptamers provide high specificity for molecular recognition.

Purpose of the Study:

  • To develop a fluorescent sensing platform using graphene oxide hydrogel.
  • To utilize adenosine and aptamer as co-crosslinkers for hydrogel formation.
  • To establish a sensitive and selective method for oxytetracycline (OTC) detection.

Main Methods:

  • Fabrication of graphene oxide hydrogel via facile gelation and immersion.
  • Incorporation of adenosine and aptamer as co-crosslinkers for 3D structure formation.
  • Fluorescence determination for quantitative analysis of oxytetracycline.

Main Results:

  • The developed hydrogel exhibited high mechanical strength and thermal stability.
  • A linear response for oxytetracycline (OTC) was observed in the range of 25-1000 μg/L.
  • The sensing platform demonstrated excellent sensitivity and selectivity with a limit of quantitation (LOQ) of 25 μg/L.

Conclusions:

  • The graphene oxide hydrogel serves as a robust and sensitive fluorescent sensing platform.
  • The platform demonstrates potential for generic detection of various molecules by replacing the recognition element.
  • This work lays a foundation for assembling functionalized hierarchical graphene-based materials for analytical applications.