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Related Experiment Video

Updated: Mar 21, 2026

Immunomagnetic Separation of Fat Depot-specific Sca1high Adipose-derived Stem Cells ASCs
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Cell Surface Markers on Adipose-Derived Stem Cells: A Systematic Review.

Alexander Mildmay-White1, Wasim Khan2

  • 1University College London Medical School, Gower Street, London, WC1E 6BT, United Kingdom.

Current Stem Cell Research & Therapy
|May 3, 2016
PubMed
Summary

This study identifies key cell surface markers for adipose-derived stem cells (ASCs). A recommended panel of positive and negative markers aids in consistent identification of these valuable stromal cells.

Keywords:
Adipose-derived stem cellcell surface characterizationmesenchymal stem cellstromal cellsurface markers

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Area of Science:

  • Stem Cell Biology
  • Cell Surface Marker Analysis

Background:

  • Adipose-derived stem cells (ASCs) are mesenchymal stem cells isolated from adipose tissue.
  • Characterization of ASCs, including their cell surface profile, is crucial for understanding their potential.
  • Focus is on identifying reliable markers for ASC identification.

Purpose of the Study:

  • To systematically review and summarize literature on ASC cell surface markers.
  • To establish a consensus on positive and negative markers for consistent ASC identification.
  • To facilitate reliable in vitro and in vivo characterization of ASCs.

Main Methods:

  • Comprehensive literature search using keywords 'adipose-derived stem cells', 'stromal cells', and 'surface markers'.
  • Databases searched include Medline, PubMed, ZETOC, Web of Knowledge, AMED, EMBASE, Ovid, and PsychINFO.
  • Analysis and synthesis of reported cell surface marker expression data.

Main Results:

  • Commonly reported positive markers include CD90, CD44, CD29, CD105, CD13, CD34, CD73, CD166, CD10, CD49e, and CD59.
  • Frequently identified negative markers are CD31, CD45, CD14, CD11b, CD34, CD19, CD56, and CD146.
  • Other markers like HLA-ABC, STRO-1, HLA-DR, c-Kit, and OCT4 were also noted, with some discrepancies.

Conclusions:

  • A minimum panel of positive (CD90, CD44, CD29, CD105, CD13, CD34, CD73, CD166, CD10, CD49e, CD59) and negative (CD31, CD45, CD14, CD11b, CD19, CD56, CD146) markers is recommended for ASC identification.
  • Positive expression of HLA-ABC and STRO-1, with negative HLA-DR, further refines identification.
  • Disagreements exist regarding markers such as CD31, CD34, c-Kit, and STRO-1, necessitating further research.